条锈菌侵染慢锈性小麦品种川麦107后的蛋白质组学分析  

Proteome Analysis of Slow-rusting Variety Chuanmai 107 Inoculated by Wheat Stripe Rust(Puccina striiformis)

作　　者：梁根云[1] 姬红丽[2] 章振羽[2] 魏会廷[2] 康振生[1] 彭云良[2] 李跃建[2] 

机构地区：[1]西北农林科技大学植物保护学院 [2]四川省农业科学院植物保护研究所,四川成都610066

出　　处：《麦类作物学报》2007年第2期335-340,共6页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30571162);四川省应用基础项目;教育部创新团队发展计划项目(IRT0558)

摘　　要：为了在基因和蛋白表达水平上研究小麦的慢条锈性机制,运用双向电泳技术分析了条锈菌侵染的2个小麦品种(川麦107和80-8)的对照和发病14 d叶片的差异表达蛋白,从2-D图谱上找到9个差异表达蛋白,其中点P5是在接种并发病的川麦107中新诱导出的一个差异蛋白质。通过基质辅助激光解析电离飞行时间质谱技术(MALDI-TOF-MS)获得6个蛋白质的肽指纹图谱(PMF),数据库搜索鉴定出2个蛋白质。这2个蛋白质是磷酸核酮糖激酶(Phosphoribulokinase,PRK)和脱氢抗坏血酸还原酶(Dehydroascorbate re-ductase,DHAR)。The changes of proteins in two wheat varieties, Chuanmail07and 80 - 8, caused by the inoculation of Puccina striiformis f. sp. tritici were analyzed using 2-dimentional electrophoresitic tech- nology. 9 protein spots resolved on 2-D gels were found to be differentially expressed after analyzing Coomas-blue stained gel of the soluble protein samples from the control and disease-affected leaves at 14 days post inoculation. These spots were then excised from gel and analysed by matrix-assisted laser desorption ionisation time of flight mass spectrometry （MALDI-TOF-MS）. Peptide mass fingerprinting （PMF） of 6 proteins were submitted to NCBI and Swiss-prot databases for protein identification. Protein spot P5 only induced in Chuanmai 107 inoculated by CY32 was an unknown protein. 2 proteins identified were respectively phosphoribulokinase （PRK） and dehydroascorbate .reductase （DHAR）. Proteome analysis of slow-rusting variety , Chuanmai 107, may provide clues for elucidating the resistant mechanisms of slow-rusting variety.

关 键 词：小麦 慢条锈性 差异表达蛋白 肽指纹图谱 蛋白质组学 

分 类 号：S512.1[农业科学—作物学] S435