不同巢穴尼科巴弓背蚁的RAPD分析  

RAPD Analysis on Different Nests of Camponotus nicobarensis

作　　者：高成伟[1] 庞广福[1] 周善义[1] 秦新民[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2007年第1期100-103,共4页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30360013);广西自然科学基金资助项目(0236015)

摘　　要：用RAPD技术对尼科巴弓背蚁5个巢穴的DNA样品进行分析,15个RAPD引物共检测到61个位点,每个引物产生2～7个位点,其中23个为多态位点,占37.70%,Nei＇s基因多样性指数H为0.122 0,Shannon多态性信息指数I为0.185 8.地域之间遗传相似性系数S的变化在0.851 5～0.948 5,遗传距离指数D的变化为0.051 6～0.160 3,用NTSYSpc 2.10软件包中的UPGMA法进行聚类分析.结果表明,5个不同巢穴间的尼科巴弓背蚁亲缘关系与地理距离相吻合.The Genetic diversity and phylogenesis of Camponotus nicobarensis Mayr from five different nests were analyzed by Random Amplified Poly-morphic DNAs （RAPD） technique. 61 RAPD markers were obtained, which were amplified by 15 random declainer primers. Among them, 23 marker with 37.70% polymorphisms were detected. The results indicate that the Nei＇s gene divesity （H）, and Shannon＇s information index（I） was 0. 122 0 and 0. 185 8,respectively. And the genetic distance among which was varied from 0. 851 5-0. 948 5,with distance coefficient between 0. 051 6-0. 160 3. Based on genetic distance and geneticsimilarity,molecular phylogenetic tree of Camponotus nicobarensis Mayr has been constructed by using UPGMA method. The results showed that genetic relationship of 5 populations of Camponotus nicobarensis Mayr correspond to geographic distance.

关 键 词：RAPD 尼科巴弓背蚁 遗传距离指数 遗传相似性系数 

分 类 号：Q966[生物学—昆虫学]