瘦素对巨噬细胞白细胞介素1β表达的影响  

The Effects of Leptin on the Expression of Interleukin-1β in Macrophages

作　　者：唐澜[1] 王丽[1] 付度关 赵勇[1] 曾和松[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,湖北省武汉市430030 [2]湖北襄樊市第一人民医院心内科,湖北省襄樊市441000

出　　处：《中国动脉硬化杂志》2006年第9期747-749,共3页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助项目(30470713)

摘　　要：目的探讨瘦素对鼠源性巨噬细胞系RAW264.7细胞白细胞介素1β表达的影响,以阐明瘦素在动脉粥样硬化发生中的作用。方法不同浓度的瘦素(1.25、2.5、5和10nmol/L)孵育RAW264.7细胞4h后,逆转录聚合酶链反应法检测巨噬细胞白细胞介素1βmRNA的表达。并用该浓度梯度的瘦素分别孵育RAW264.7细胞1、3、6、9h后,采用双抗夹心酶联免疫吸附法检测各组培养上清白细胞介素1β蛋白含量。结果白细胞介素1βmRNA表达水平随瘦素浓度增加逐渐增高(分别为0.107±0.102、0.204±0.019、0.718±0.083、0.642±0.071),5nmol/L瘦素处理组达最高峰。各组培养上清白细胞介素1β蛋白分泌水平随瘦素浓度增加逐渐增高,5nmol/L瘦素处理组白细胞介素1β蛋白分泌量达最高峰;白细胞介素1β蛋白分泌水平随瘦素刺激时间增加逐渐增高,至6h达最高峰(P<0.05)。RAW264.7细胞经不同浓度瘦素处理后,白细胞介素1βmRNA和蛋白表达呈瘦素剂量依赖性增加。结论瘦素可在体外促进RAW264.7细胞白细胞介素1β表达和分泌,这可能是瘦素直接致动脉粥样硬化的机制之一。Aim To investigate the effects of leptin on the expression of intedeukin- 1β （ IL-1β） in macrophages in vitro. Methods BAW264.7 cells were incubated with leptin in different concentrations （ 1.25, 2.5, 5, 10 nmol/L） for 4 hours, reverse transcription polymerase chain reaction （RT-PCR） was used to measunre the expression of IL-1β mRNA level; Meanwhile, RAW264.7 cell were incubated with these concentrations of leptin for 1, 3, 6 and 9 hours, then the expression of IL-1β in the medium was detemined by enzyme linked immunosorbent assay （ ELISA）. Results The expression of IL- 1β mRNA was promoted with the concentration of leptin （0.107 ±0.102, 0.204±0.019, 0.718±0.083, 0.642±0.071）, and reached the maximum level at 5 nmol/L of leptin. At the same time , the expression of IL-1β protein was proted with the concentration and the time time of leptin stimulating ,and reached the maximmn at 6th hour（P〈0.05）. The expression of IL-1β in both mRNA and pro tein levels in RAW＇264.7 cells incubated with leptiin were increased with the leptin concentration, Conclusion Leptin can in-crease the IL- 1β expr ession a RAW264.7 cells-directly and that might be one of mechanism of atherosclerosis induced by leptin.

关 键 词：内科学 瘦素 白细胞介素1Β 细胞培养 动脉粥样硬化 巨噬细胞 

分 类 号：R5[医药卫生—内科学]