沼泽红假单胞菌中类胡萝卜素的提取与分析  

Study on Caroenoids Extraction and Analysis from Rhodopseudomansa

作　　者：李福枝[1] 刘飞[1] 邓靖[1] 

机构地区：[1]湖南工业大学绿色包装与生物纳米技术应用重点实验室,湖南株洲412008

出　　处：《生物技术》2007年第1期50-53,共4页Biotechnology

基　　金：湖南省教育厅项目资助("红螺菌深层发酵培养技术";06C258)

摘　　要：目的:研究沼泽红假单胞菌中类胡萝卜素的提取工艺条件。方法:对细胞收集、前处理、色素初浸、二次萃取,皂化和溶剂回收等方面进行了系统的研究,并通过紫外分光光度法和高效液相色谱法对所提取的类胡萝卜素进行了分析。结果:实验结果表明,类胡萝卜素的最佳提取工艺条件为:碱性氯化钙絮凝法收集细胞,乙醇浸泡法进行前处理,49℃下丙酮初浸提浸提4h(搅拌并加抗氧化剂),丙酮用量为5mL/g菌泥,回收丙酮,乙醚二次萃取,30℃下皂化1.5h,洗涤,浓缩并回收乙醚,在此提取工艺条件下不仅能够有效地提取红螺菌中的类胡萝卜素,而且还能分离细菌叶绿素。结论在本实验条件下,根据标准曲线计算出类胡萝卜素的含量高达6.148mg/L菌泥。通过HPLC分析表明:类胡萝卜素提取液中主要成分至少有10种。Objective：In order to study the conditions for carotenoids extraction from Rhodopseudomansa,the cell collection,cell pretreatment,first extraction,second extraction,saponification and solvent callback were researched.And the analysis about carotenoids was done by uv-vis spectrophotometer and HPLC.Result：As results,the best extraction conditions were： cell collection with alkaline calcium chloride,pretreatment with ethanol,first extraction with acetone at 49 ℃ for 4h（5mL acetone：1g bacteria mud）, solvent reclaim,second extraction,saponification at 30℃ for 1.5 hours,washing,condensation and solvent reclaim.Not only the carotenoids but also the chlorophylls were gained under the condition.The total content of carotenoids was high to 6.18mg/L on the basis of lycopene calibration curve,and ten kinds of carotenoids were found in the extraction by HPLC.

关 键 词：沼泽红假单胞菌 类胡萝卜素 叶绿素 前处理 皂化 

分 类 号：Q939.97[生物学—微生物学]