无患子愈伤组织诱导的多因子正交试验研究  

Studies on Callus Induction in Sopindus mukorossi Gaertn by Orthogonal Design

作　　者：陈光蓉[1] 殷家明[1] 张凤龙[2] 谢必武[2] 卿励[2] 

机构地区：[1]西南大学农学与生命科学学院,重庆400716 [2]重庆三峡职业学院生物工程系,重庆404001

出　　处：《生物技术》2007年第1期78-81,共4页Biotechnology

基　　金：重庆市万州区重点科技项目资助("无患子的生物学特性及快繁研究";编号:2004102)

摘　　要：目的：探寻无患子愈伤组织诱导的最佳外植体、培养基和培养条件.方法：采用5因素混合水平正交设计,用DPS2000统计软件对其结果进行方差分析和多重比较（SSR）.结果：诱导愈伤组织的最佳外植体为当年生枝条水培芽的嫩茎段,用0.2%HgCl2消毒2min,其无菌存活率为98.33%,培养条件为光照或黑暗,最佳培养基配方为：MS（B5）＋6-BA 1.5mg·l^-1＋NAA0.2mg·l^-1,其愈伤组织的诱导率为97.78%.结论：激素配比是影响愈伤组织诱导的主要因素.较高的6-BA浓度（1.5～2.5 mg·l^-1）与较低的NAA浓度（0.2mg·l^-1）配比,有利于无患子愈伤组织的诱导.Objective：It was to search after the optimal explants,medium and condition of callus inducement of Sopindus mukorossi Gaertn.Methods： The Orthogonal design L8（4×2^4） was used in this experiment and the statistic software DPS2000 was employed to analyse the result＇s variance and multiple comparisons.Results： The results showed that the tender stem on the twig which grew in sterile water was best explants,which was sterilized with 0.2% HgCl2 for 2min.Its surviving rate was 98.33%;Inducing condition was darkness or light; the optimal medium was MS （B5） ＋ 6- BA 1.5mg·l^-1 ＋ NAA 0.2mg·l^-1 , On which callus induction rate was 97.78%. Conclusiou： The influence of the combination of 6 - BA and NAA on callus induction was a key. A considerable 6- BA concentrafion（1.5 ～2.5mg·l^-1 ）in combination with a little NAA（0.2mg·l^ -1 ） would avail callus induction.

关 键 词：正交设计 外植体 存活率 愈伤组织 诱导 

分 类 号：Q813.2[生物学—生物工程]