超级稻沈农265、沈农606成熟胚愈伤组织诱导的研究  

Callus Induction from Mature Seeds of Super Rice Shennong-265 and Shengnong-606

作　　者：胡凯[1] 张立军[1] 崔震海[1] 白雪梅[1] 崔玉娜[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110161

出　　处：《生物技术》2007年第1期81-85,共5页Biotechnology

摘　　要：超级稻沈农265、沈农606具有高产、优质等特点,是通过基因工程进行遗传改良的良好基础材料,该文以这两个品种的成熟胚为材料研究了这的愈伤组织诱导条件,以便为进一步转基因的工作奠定基础。结果表明:以MS基本培养基为诱导培养基,其中添加3mg/L 2,4—D、30g/L蔗糖、0.8%琼脂,pH为5.8,在27℃条件下培养30d,能够诱导出质量较好的愈伤组织,诱导率均可以达到90%以上。研究还表明生长素2,4—D对愈伤组织的诱导起着主要作用;适量的6-BA和ABA对愈伤组织的诱导起到一定的积极作用,但效果不明显;蔗糖浓度高于40g/L时,诱导率急剧下降;愈伤组织的诱导受温度的影响很大;而有无光照对愈伤组织的诱导没有影响。Super rice-shennong-265 and shennong-606 have the trait of high yield and good quality,and they are good material for genetic improvement by gene transfer.In order to study of the gene transfer,in the article we studied the optimization of the conditions for an efficient callus induction from mature seeds of shennong-265 and shennong-606.The results showed that： callus induction was most efficient in MS medium with 3mg/L of 2,4-D,30g/L of sucrose and 0.8% agar,pH 5.8,in the condition of 27℃, and callus induction rotes were above 90%. Moreover, the results also showed that： 2,4- D was critical for callus induction of rice mature seeds; proper level of 6- BA and ABA advanced callus induction, but the affect wasn＇t distinct; when sucrose concentration above 40g/l, the callus induction was inhibited; callus induction was affected distinetly by the temperature, but was not affected by the light.

关 键 词：超级稻 成熟胚 愈伤组织 激素 温度 

分 类 号：Q813.1[生物学—生物工程]