乌司他丁对高原犬体外循环肾功能的保护作用  

Protective effect of ulinastatin on renal function in dogs undergoing cardiopulmonary bypass at high altitude

作　　者：刘汉斌[1] 李素芝[1] 张安生[2] 易映红[1] 

机构地区：[1]西藏军区总医院泌尿外科,拉萨850000 [2]武警总部门诊部,北京100089

出　　处：《武警医学》2007年第4期266-269,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨乌司他丁在高原犬体外循环中对肾损伤的保护作用。方法高原雄性健康成年杂种犬24只,随机分为3组:对照组(C组)、体外循环组(I组)、乌司他丁组(U组),各组在转流结束后0、2、6、12、24 h留取血、尿标本,分别测定血尿素氮(BUN)、血肌酐(Cr)、尿N-乙酰-β-D-氨基葡萄糖苷酶(N-acetyl-β-D-glucosaminidase,NAG)和尿γ-谷氨酰转肽酶(γ-GTP)。结果与C组比较,I组尿NAG和γ-GTP活性在各时相点增高显著,血清BUN、Cr浓度仅在122、4 h增高显著(P<0.05);U组在各时相点尿NAG活性增高明显、尿γ-GTP活性在01、2、24 h增高明显(P<0.05),血清BUN、Cr浓度增高在各时相点差异均无统计学意义(P>0.05)。与I组比较,U组尿NAG活性在各时相点降低明显(P<0.05)、尿γ-GTP活性在6、122、4 h明显降低(P<0.05),U组血清BUN、Cr浓度仅在24 h降低有统计学意义(P<0.05)。结论乌司他丁对高原体外循环犬肾功能有保护作用,主要与减轻肾脏缺血再灌注损伤(Ischemical reperfusion injury,IRI)有关。Objective To investigate the effects of ulinastatin on renal function in dogs undergoing cardiopulmonary bypass（CPB） at high altitude. Methods Twenty- four male healthy dogs at high altitude were randomly divided into three groups with 8 animals in each group： 1 ） control group （ C ） received sham operation; 2 ） isehemic reperfusion renal injury group （ I ）, and （ 3 ） ulinastatin group （ U ）. Blood and urine samples were obtained at 0, 2, 6, 12, 24 h after CPB for determination of blood urea nitrogen （BUN）, serum creatinine （Cr） concentration, urine N - acetyl - β - D - glucosaminidase （NAG）, and urine glutamyl transpeptidase （γ- GTP） .Results At 0, 2, 6, 12, 24 h after CPB, BUN and Cr levels in groups I were significantly higher than in group （C） （ P 〈 0.05）, but those in group U were not significantly higher （ P 〉 0.05）; NAG levels in group U and in group I were significantly higher （ P 〈 0.05）. γ- GTP levels at 0,2,6, 12,24 h after CPB in group I were significantly higher （ P 〈 0.05） γ- GTP levels at 0, 12, 24 h after CPB in group U were significantly higher （ P 〈 0.05）. Compared with group I, BUN and Cr levels in group U were significantly low at 24 h after CPB （ P 〈 0.05）, NAG levels in group U were significantly low at 0, 2, 6, 12, 24 h after CPB （ P 〈0.05）, γ- GTP levels in group U were significantly low at 6, 12, 24 h after CPB （ P 〈 0.05）. Conclusions Ulinastatin reduces the risk of renal dysfunction and injury during CPB by attenuating renal ischemical reperfusion injury（IRI）.

关 键 词：高原 体外循环 乌司他丁 肾脏缺血再灌注损伤 

分 类 号：R96[医药卫生—药理学]