一种肝癌特异性GGT的DSA亲和色谱检测方法  

Hepatoma-Specific Glutamyltranspeptidase Detected by DSA Affinity Chromatography Method

作　　者：蒋淑莲[1] 王念跃[1] 文剑[1] 赵伟[1] 吴引伟[1] 

机构地区：[1]东南大学医学院附属南京第二医院,江苏省南京市210003

出　　处：《中国全科医学》2007年第7期533-535,共3页Chinese General Practice

基　　金：江苏省自然科学基金项目(BK2004013);南京市医学重点科技项目(ZKX0401)

摘　　要：目的建立一种特异的检测血清中肝癌特异性γ-谷氨酰转移酶(HS-GGT)的亲和层析方法,并观察HS-GGT对原发性肝癌(PHC)的鉴别诊断价值。方法采集64例PHC、22例肝外肿瘤、68例良性肝病及21例正常对照者的血清,以神经胺酸酶(NMD)水解后直接用欧曼陀罗凝集素(DSA)亲和层析柱分离,首先用柱平衡液洗脱不结合组分,再用50mmol/L醋酸洗弱结合组分,最后用100mmol/L醋酸洗脱强结合组分,用连续监测法测定各组分中的GGT活性,并分析各组分GGT在PHC鉴别诊断中的价值。结果DSA强结合组分中的GGT为PHC患者所特有,以其在总GGT所占比例>2%为PHC诊断的Cutoff值,其诊断特异度为95.5%,灵敏度为85.8%,准确度为92.0%。结论该方法能较好地鉴别诊断PHC,较其他检测方法更为简便,且同样有着较高的诊断灵敏度和特异度。Objective A simple method for detection of hepatoma - specific gamma - glutamyhranspeptidase （ HS - GGT） was set upby Datura stramonium agglutinin （DSA） affinity chromatography, and its clinical application was verified in differential diagnosis of primary hepatocellular carcinoma （PHC）. Methods Serums were collected from 64 patients with PHC, 22 with extrahepatic tumor, 68 with benign liver diseases and 21 normal controls; and then they were analyzed by DSA - sepharose affinity chromatography after digestion by neuraminidase. Three fractions were eluted and collected by equilibration buffer, 50 mmol/L acetate and 100 mmol/L acetate respectively. GGT activity in each fraction was tested by continuous monitoring method, and the differential diagnostic value of GGT in each fraction was assessed. Results DSA strongly bound GGT Ⅲ was specific in PHC patients, when the proportion of GGT Ⅲ〉 2% was taken as the cutoff value, the specificity, sensitivity and accuracy of GGT Ⅲ in diagnosis of PHC were respectively 95.5%, 85.8% and 92. 0%. Conclusion The method was helpful for differential diagnosis of PHC, simpler and with similar high sensitivity and specificity as compared to other methods.

关 键 词：亲和层析 Γ-谷氨酰转移酶 肝细胞癌 欧曼陀罗凝集素 

分 类 号：R446[医药卫生—诊断学] R735.7[医药卫生—临床医学]