过氧化物模拟酶催化荧光反应性能的研究——FeTMPvP-HVA-H_2O_2体系  

Studies on Catalytic Fluorescence Reaction by Mimetic Enzyme of Peroxidase—Ferric Tetrakis (N-Methylpyridiniumyl) Porphyrin-Homovanillic Acid-H_2O_2 System

作　　者：慈云祥[1] 王芳[1] 

机构地区：[1]北京大学化学系,北京100871

出　　处：《分析化学》1990年第4期334-338,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：本文研究了moso-四(N-甲基-3-吡啶基)卟啉和铁的络合物作为辣根过氧化物酶的模拟酶催化过氧化氢氧化高香草酸的荧光反应的性能。拟订了用模拟酶催化测定H_2O_2和葡萄糖的荧光测定方法。其检出下限分别为1.1×10^(-7)mol/LH_2O_2和0.2μgml^(-1)葡萄糖。对血清中葡萄糖的含量进行了测定,结果令人满意。通过动力学研究,比较了该模拟酶与辣根过氧化物酶及其它模拟酶催化活性的相对大小。The catalytic behaviours of the mimetic enzyme of peroxidase, ferric tetrakis(N-methyplyridiniumyl)porphyrin(FeTMPyP), have been demonstrated on the fluorescence reaction of homovanillic acid with hydrogen peroxide. The lowest limits of the determination of hydrogen peroxide and glucose are 1. 1×10^(-7)mol/L and 0. 20μg/ml, respectively. The recommended method has been applied to determine glucose in human serum and the satisfactory result has been obtained. The catalytic activity of FeTMPyP has been compared with those of HRP and other mimetic enzymes.

关 键 词：模拟酶 铁卟啉 葡萄糖 血清 

分 类 号：R446.112[医药卫生—诊断学]