过氧化物模拟酶的合成及在血清葡萄糖测定中的应用  

Synthesis of Mimetic Peroxidase and Its Application for the Determination of Glucose in Serum

作　　者：孙淑声[1] 郑智平 

机构地区：[1]北京大学化学系,北京100871

出　　处：《分析化学》1990年第4期329-333,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：本文合成了锰(Ⅱ)与meso-四(3-N-甲基吡啶基)卟啉的螯合物Mn-T(3MPy)P,研究了其作为过氧化物模拟酶对过氧化氢、4-氨基安替比林与苯酚显色反应的催化活性。拟定的催化光度法可以准确地测定0～5.5μg/ml范围内H_2O_2含量。将显色反应与葡萄糖氧化酶催化反应偶联,用于测定人血清中的葡萄糖,取得了满意的结果。A chelate compound of manganese tetra(3-N-methylpyridyl)-porphyrin〔(Mn-T(3MPy)P〕has been synthesized and its catalytic characteristics as a mimetic peroxidase in the chromogenic reaction of hydrogen peroxide with 4-aminoantipyrine and phenol have been studied. With the mimetic catalytic reaction, trace of hydrogen peroxide can be determined spectrophotometrically with good accuracy and precision. By coupling this reaction with the catalytic reaction of glucose oxidase, glucose in human serum has been determined with satisfactory results.

关 键 词：模拟酶 催化光度法 血清 葡萄糖 

分 类 号：R446.112[医药卫生—诊断学]