猪笼草组培快繁技术研究  

Tissue culture and rapid propagation of Nepenthes mirabilis

作　　者：谢怡青[1] 谢雄辉[1] 丘亮伟[1] 黄愉光[1] 

机构地区：[1]梅州市农业科学研究所,广东梅州514011

出　　处：《广西农业科学》2007年第2期131-132,共2页Guangxi Agricultural Sciences

摘　　要：以猪笼草的顶芽和腋芽为外植体进行组培快繁研究,结果表明:在1/2MS+6-BA 1mg/L+NAA 0.1mg/L培养基中培养10d后,能诱导出幼芽;1/2MS+6-BA 2mg/L+NAA 0.1mg/L培养基对丛生芽的增殖效果最好,增殖倍数达4.1;生根培养基以1/4MS+6-BA 0.1mg/L+NAA 0.5mg/L为最佳,生根率可达95%以上。The top buds and axillary buds of Nepenthes mirabilis used as explants for tissue culture and rapid propagation were studied. The results showed that the young buds could be induced after 10 days cultured on the medium 1/2MS＋6-BA lmg/L＋NAA 0. lmg/L. The 1/2MS medium with 6-BA 2. 0mg/L and NAA 0. lmg/L was the best for multiplication culture of clumpy buds and the multiplication coefficient was 4. 10. The 1/4MS medium with 6-BA0. 1mg/L and NAA 0.5mg/L was the best for inducing roots from shoots and the rootage ratio was above 95 %.

关 键 词：猪笼草 组织培养 快速繁殖 

分 类 号：Q949.749.3[生物学—植物学] S336[农业科学—作物遗传育种]