褪黑素对谷氨酸致痫大鼠脑内cGMP水平的影响  

Effects of Melatonin on Contents of Hippocampal Cyclic GMP in L-Glutamate-induced Seizures in Rats

作　　者：周厚纶[1] 袁远[1] 卢敏[1] 魏瑛[1] 

机构地区：[1]华中科技大学同济医学院基础医学院解剖学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2007年第2期149-152,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨褪黑素(melatonin,MT)对谷氨酸(glutamate,Glu)致痫大鼠海马cGMP水平的影响。方法将健康SD雄性大鼠随机分为A、B、C、D 4组,每组10只,分别为对照组、Glu组、MT+Glu组和Luzidole+MT+Glu组。观察并记录动物行为学及脑电图(EEG)改变,应用放射免疫方法检测各组动物脑内cGMP水平。结果行为学观察和EEG显示,B组和D组大鼠均出现痫性发作,并出现频发性痫性放电,C组大鼠痫性发作不明显,无频发性痫性放电出现;放射免疫分析结果显示,与A组比较,B、C、D组海马cGMP含量显著升高(均P<0.05);C组较B组和D组cGMP水平明显降低(P<0.05)。结论MT对Glu致痫有抑制作用,此作用可能是通过MT受体作用于cGMP实现的。Objective To investigate the effects of melatonin on contents of hippocampal cyclic GMP in L-glutamate （Glu）- induced seizures in rats. Methods Forty healthy male SD rats were randomly divided into 4 groups （n： 10 in each group） ：control group （group A）, Glu group （group B）, MT＋Glu group （group C） and Luzidole＋MT＋Glu group （group D）. The changes in behaviors and EEG were observed and recorded, and cyclic GMP in brain was determined by using radioimmunoassay. Results Observation on behaviors and EEG revealed that the rats in groups B and D had seizure onsets and frequent seizure discharge. But in group C, the seizure onset was not obvious and no frequent seizure discharge occurred. Radioimmunoassay showed that the cGMP levels in hippocampus of groups B, C and D were significantly higher than in group A （P〈0.05）, while the cGMP levels were significantly decreased in group C as compared with groups B and D （P〈0.05）. Conclusion Melatonin may prevent Glu-induced seizures in rats, and cGMP may play a role in this procedure.

关 键 词：癫痫 环磷酸鸟苷 谷氨酸 褪黑素 海马 

分 类 号：R322.811[医药卫生—人体解剖和组织胚胎学]