肺血栓栓塞症中细胞因子的变化及当归注射液对其的影响  

The expression levels of ET-1,COX-2 in the lung tissue of acute pulmonary embolism and the effects of angelica

作　　者：高岩[1] 张凌[1] 夏蕾[1] 白明[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院呼吸内科,武汉430022

出　　处：《华中医学杂志》2007年第2期102-104,共3页Central China Medical Journal

摘　　要：目的观察内皮素-1(ET-1)、环氧合酶-2(COX-2)在肺内的表达及血浆中血栓烷素B2(TXB2)和6-酮-前列腺素F1α(6-K-PGF1α)的比值(T/P)变化,并探讨当归注射液对其影响。方法采用自体血栓回输法建立动物模型,将SD大鼠随机分为正常组、肺血栓栓塞症(PTE)模型组和当归治疗组。行常规病理检查,免疫组化法检测肺内ET-1、COX-2蛋白表达水平,放射免疫法测定血浆TXB2、6-K-PGF1α浓度变化。结果PTE模型组肺组织炎症损伤明显,当归治疗组损伤较轻。ET-1、COX-2蛋白表达水平:PTE模型组较正常组高(P<0.05);当归治疗组较PTE模型组低(P<0.05)。放免检测T/P:PTE模型组明显较正常组高(P<0.05);当归治疗后组T/P与正常组间差异无显著性(P>0.05)。结论当归辅助治疗可明显减少急性肺血栓栓塞症大鼠ET-1、COX-2的表达,调节T/P比例,改善病情。Objective To study the expression levels of endothelin-1 （ET-1）, cyclooxygenase-2 （COX-2） in the lung tissue, the concentrations of thromboxane B2 （TXB2） and 6-keto- prostaglandin F1α（6-K-PGF1α） in the plasma in acute pulmonary thromboembolism（PTE）, and to explore the effects of angelica injection on their expressions. Methods Rats were randomly divuded into a blank group, a PTE model group, a angelica injection treat group. The PTE model was established by intravenous injection of autologous blood clots. The pathological changes of the lungs were examined with light microscope. By using immunohistochemistry, the expression levels of ET-1, COX-2 in lung tissue were examined. The plasma＇s concentrations of TXB2 and 6-K-PGF1α were examined by radioimmunity assay. Results Histopatbological study showed severe lung injury in the PTE group, but mild in the angelica injection group. Immunohistochemistry study showed the difference between the blank group and the PTE group, the PTE group and the angelica group was significant （P〈0. 05）. The rate of TXB2 and 6-K-PGF1α＇s concentrations （T/P）in the plasma study showed the difference between the blank group and the PTE group was significant （P〈 0. 05）. T/P difference between the blank group and the angelica group was slight（P〉0. 05）. Conclusion Angelica injection can reduce the expression levels of ET-1 ,COX-2 in acute PTE.

关 键 词：肺栓塞 当归注射液 内皮素-1 环氧合酶-2 大鼠 

分 类 号：R285.5[医药卫生—中药学]