超高灵敏显色反应及其应用的研究——Ⅳ.生命活性元素锌的高灵敏光度法  

Studies on Extra-High Sensitive Colour Reaction and Their Applications Ⅳ.High Sensitive Spectrophotometric Determination of Trace Zinc

作　　者：滕毓敏[1] 许生杰 

机构地区：[1]中国科学院长春应用化学研究所

出　　处：《分析化学》1990年第6期515-519,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助课题

摘　　要：本文研究了Zn^(2+)-SCN^--RB^+-PVA_(124)超高灵敏显色体系及锌的分光光度测定条件。缔合物的吸收峰在607nm处,表观摩尔吸光系数达2.6×10~6L·mol^(-1)·cm^(-1)。Zn^(2+)浓度在0～0.5μg/25ml范围内符合比耳定律。测得缔合物的组成比Zn^(2+):RB^(+)为1:30。初步研讨了显色反应的机理。方法有较好的选择性,可不经预分离,成功地用于水样,麦饭石浸出液、花粉和发样中痕量锌的测定。The colour system of Zn^(2+)-SCN^--rhodamine B(RB)-PVA^(124) has been studied. In the medium of hydrochloric acid, the complicated ion-association complex of Zn^(2+)with RB and thiocyanate exhibits maximum absorption at 580nm. Its apparent molar absorptivity is 2. 6×10~6 L·mol^(-1)·cm^(-1). Beer＇s law is obeyed for zinc in the range of 0～0. 5μg/25ml. The reaction mechanism has been discussed and the molar ratio of Zn to RB in ion-associate was found to be 1: 30 as estabilished by molar ratio method and molar absorptivity contrast method. The proposed method has been used for determination of trace Zn in water, leaching solution of Maifan stone, pollen sample and hair with satisfactory results.

关 键 词：锌 罗丹明B 分光光度法 

分 类 号：O614.241[理学—无机化学]