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作 者:胡蓉[1] 李春苗[1] 黄伟[1] 徐华伟[1] 唐正义[1]
机构地区:[1]内江师范学院化学与生命科学系,四川内江641112
出 处:《内江师范学院学报》2007年第2期64-66,共3页Journal of Neijiang Normal University
基 金:内江师范学院自然科学类资助项目(No:04NJZ-03);内江师范学院大学生科研项目(No:05NSD-096)
摘 要:通过石蜡切片,汞-溴酚蓝染色,在显微镜下系统观察了川白芷胚胎发育时期蛋白质的动态变化.结果表明:在受精后1-16d,蛋白质积累逐渐增多,且胚细胞中的蛋白质含量的增加明显快于珠被;合点端细胞中蛋白质很明显,珠孔端蛋白质少于合点端;第17d后,胚和珠被的蛋白质含量逐渐下降.Dynamic variation of proteins during embryo developing in Angelica dahurica (Fishch) Benth. et Hook was observed by the numbers under optical microscope (OLMPUS-CX21) by paraffin slice and Mercury-bromophenol blue dyeing. The results suggested that the accumulation of protein increased gradually from 1 to 16 day after fertilization and the increase of protein in the cell of embryo sac was apparently quicker than in the carrinuceller;the protein in the cell of chalaza was very clear and the protein at micropyle was fewer than chalaza; the content of protein in embryo sac and carrinuceller decreased gradually after 17 days.
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