PGE_1对血小板的体外激活抑制作用  

Inhibitory of PGE_1 on Platelet Activation in Vitro

作　　者：周俊[1] 刘景汉[2] 邢颜超[1] 王冬梅[1] 

机构地区：[1]兰州军区乌鲁木齐总医院输血科,830000 [2]中国人民解放军北京军区总医院输血科

出　　处：《临床输血与检验》2007年第2期101-105,共5页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：国家自然科学基金资助(No.30271443)

摘　　要：目的研究前列腺素E1(PGE1)在血小板冻干前预处理过程中对血小板激活和功能的影响,为血小板冻干前处理过程筛选血小板激活损伤保护剂。方法测定血小板平均体积(MPV),采用流式细胞术(FCMs)分析血小板CD62p、PAC-1的表达,以反映血小板状态。测定血小板对凝血酶(thrombin)、二磷酸腺苷(ADP)和瑞斯托菌素(Restocetin)的最大聚集率,以反映血小板功能,研究血小板预处理前后的变化以及前列腺素的保护作用。结果预处理后,血小板平均体积(MPV)未发生明显改变,但血小板CD62p表达率显著增加,达20%。PGE1抑制血小板CD62p表达,这种抑制作用随PGE1浓度增加而递增。预处理过程对Restocetin和ADP诱导的血小板聚集有一定影响,聚集抑制率为10%和23%,对凝血酶诱导的聚集抑制不显著。PGE1不影响血小板对Restocetin的聚集反应,但PGE1≥1μmol/L可抑制血小板对ADP的聚集反应,PGE1≥5μmol/L时,显著抑制凝血酶诱导的血小板聚集。结论前列腺素E1浓度为1μmol/L,可抑制血小板在预处理过程中的激活损伤,且保留血小板的聚集功能。Objective In order to select reversible activation inhibitors for platelets before lyophilization,effect of prostaglandin E1 （PGE1） on platelets during pre-treating was investigated. Methods MPV was detected by automatic hemanalysis. Expressions of CD62p and PAC-1 on platelet were investigated by Flow cytometry （FCMs）. The maximal aggregation of platelets was determined by platelet＇s inducers including thrombin, ADP and restocetin. Function and status of platelets were observed using above-mentioned methods before and after pre-treating with PGE1. Results After pre-treat- ing,MPV didn＇t change and CD62p expressions increased and reached 20%. PGE1 could inhibit CD62p expressing correlatively with concentration. Pre-treating affected platelets aggregation induced by resocetin and ADP, the inhibitory rates were 10% and 23% respectively. The effect on aggregation induced by thrombin was not obvious. PGE1 showed no action on aggregation induced by restocetin,but inhibited significantly aggregation induced by ADP and thrombin when 1 μmol/L and 5μmol/L PGElwere used respectively. Conclusions 1 μmol/L of PGE1 could inhibited platelet activation during pretreating, and aggregation ability was retained.

关 键 词：前列腺素E1 血小板激活 血小板冻干 

分 类 号：R331.143[医药卫生—人体生理学]