耐甲氧西林金黄色葡萄球菌的耐药性及分子流行病学调查  

Antibiotic resistance and molecular epidemiology survey of methicillin-resistant Staphylococcus aureus

作　　者：熊薇[1] 王洪波[1] 涂敏[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院医院感染管理科,武汉430030

出　　处：《中国感染与化疗杂志》2007年第2期119-123,共5页Chinese Journal of Infection and Chemotherapy

摘　　要：目的了解MRSA的耐药性及分子流行病学特点。方法采用纸片扩散法检测住院患者分离的160株MRSA的耐药性;采用随机引物(AP)-PCR法对其中的36株MRSA作同源性分析。结果160株MRSA对万古霉素、替考拉宁和复方磺胺甲噁唑的敏感率分别为100%、100%和95%;对红霉素、庆大霉素和左氧氟沙星的耐药率分别为97.5%、87%和59%;左氧氟沙星的中介率为26%。36株MRSA的AP-PCR图谱有10种类型(A～J型),主要型别为A型12株、B型7株及C型6株,ICU的25株,分别为A型10株、B型6株、C型3株、D型3株及F、I、J各1株。结论医院获得性MRSA是多重耐药菌,ICU的MRSA菌株主要基因型为A、B型。Objective To investigate the antibiotic resistance pattern and molecular epidemiology profile of methicillin-resistant Staphylococcus aureus （MRSA）. Methods Antibiotic resistance of 160 MRSA strains isolated from clinical specimens in 2004 was determined by agar diffusion. Thirty-six of the 160 MRSA strains were typed by arbitrarily primed PCR （AP-PCR）. Results All MRSA isolates were susceptible to vancomycin and teicoplanin. About 95 % of the strains were susceptible to trime- thoprim-sulfamethoxazole. However, most of the 160 MRSA strains were resistant to erythromycin （97. 5 %）, gentamicin （87%） and levofloxacin （59%）. Ten different AP-PCR patterns （A-J） were found in the 36 MRSA strains. Most of MRSA belonged to type A （n = 12）, B （n = 7） or C （n = 6）. The 25 MRSA strains from ICU were classified as type A （n = 10）, B （n = 6）, C （n = 3）, D （n = 3） and types F, I and J （one each）. Conclusions Hospital acquired MRSA is multi-resistant to antibiotics. Genotypes A and B are prevalent MRSA in ICU.

关 键 词：耐甲氧西林金黄色葡萄球菌 随机引物-聚合酶链反应 

分 类 号：R181.3[医药卫生—流行病学]