大鼠胶原诱导性关节炎早期外周血TNF-α与性激素水平的动态观察及相关性研究  

Variation of TNF-α and Sex Hormone in Periperal Blood of Collagen-induced Arthritis during the Early Stage and Correlation between These Factors in Rats

作　　者：申良红[1] 李彩萍[1] 陈丽佳[1] 

机构地区：[1]石河子大学第一附属医院,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2007年第1期81-84,共4页Journal of Shihezi University(Natural Science)

摘　　要：同步观察大鼠胶原诱导性关节炎早期血浆肿瘤坏死因子-α(TNF-α)与性激素水平的变化,探讨TNF-α和性激素在类风湿关节炎(RA)发病中的作用及其相关性。用Ⅱ型胶原与完全弗氏佐剂诱导SD大鼠胶原性关节炎(CIA),检测CIA大鼠不同时间点的血浆TNF-α、雌二醇和睾酮,同时观察其发病时间、滑膜新生血管形成、关节炎指数评分。随着CIA免疫时间的延长,滑膜轻度增厚,滑膜新生血管开始增多,关节炎指数评分渐增加,TNF-α含量也升高,睾酮水平下降,雌二醇水平无明显变化;TNF-α含量与睾酮水平呈负相关关系(r=-0.364,P<0.05),血浆TNF-α含量与雌二醇水平无明显相关关系(r=-0.209,P>0.05)。TNF-α在RA炎症反应的细胞因子网络中起重要作用,性激素与RA的发病也有密切关系,雄激素是天然的抗炎激素,雌激素的作用可能与其浓度有关。Objective to：investigate the pathogenetic roles of tumor necrosis factor-α （TNF-α）and sex hormone and the correlation between these factors in collagen-induced arthritis during the early stage. Methods： Collagen Ⅱ （ C Ⅱ） and complete Freund＇ s adjuvant were used to induce experimental arthritis, plasma contents of TNF-α, estradiol and testosterone were assayed simultaneously at different time points, and changes in beth new synovium vascularization and arthritis-index were observed along the time course. Results With the time lasting, synovium thickened lightly, synovium new vascular started to proliferate,arthritis index rose gradually, and TNF-α rose too, but testosterone was significantly lower than that of the controls, the level of estradiol was no difference between the two groups. Between testosterone and TNF-α, there was negative correlation（ r = -0.364, P 〈 0.05）, those of TNF-α and estradiol had no evidently significant correlation（ r = - 0.209, P 〉 0.05）. Conclusion： TNF-α plays important roles in RA response and in the cytokine web and sex homones also affect RA pathogenesis, andrngens seem to represent natural antiinflammatory hormones;the effect of E2 seem to be partially related to its concentration.

关 键 词：类风湿关节炎 TNF-Α 性激素 大鼠 

分 类 号：R593.9[医药卫生—内科学] R593.22[医药卫生—临床医学]