大鼠心肌细胞条件培养基对兔胚胎干细胞分离培养效果的影响  

Effect on isolation and culture of rabbit embryonic stem cells with rat cardiomyocyte conditioned medium

作　　者：郭志林[1,2] 王新庄 张涌[1] 王木[3,4] 王轶敏 胡文举[1,2] 

机构地区：[1]西北农林科技大学动物科技学院 [2]河南省肉牛工程技术研究中心,河南郑州450003 [3]河南省肉牛工程技术研究中心 [4]河南农业大学牧医工程学院,河南郑州450002

出　　处：《西北农林科技大学学报（自然科学版）》2007年第4期1-5,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河南省杰出人才创新基金项目(0421002100)

摘　　要：采用大鼠心肌细胞条件培养基对兔胚胎干细胞(Embryonic stem cells,ESC)进行分离、传代培养,研究大鼠心肌细胞条件培养基对兔ESC分离培养效果的影响。结果显示,用SD大鼠心肌细胞条件培养基培养的兔ESC集落呈岛屿状生长,碱性磷酸酶染色呈强阳性,体外分化可形成类胚体状结构,贴壁的类胚体周边会出现许多分化的上皮样细胞或单个散在的细胞;常规冻存后再传代的兔ESC集落具有较为一致的生长特征,并呈现胚胎干细胞集落所特有的岛屿状生长形态;传至第5代的兔ESC集落核型正常率>75％。表明SD大鼠心肌细胞条件培养基可用于兔胚胎干细胞分离培养。Effect on isolation and culture of rabbit embryonic stem （ES） cells with rat cardiomyocyte conditioned medium was studied. Results showed that isolated rabbit ES-like cells clusters cultured by SD rat cardiomyocyte conditioned medium were island-like;alkaline phosphatase staining of rabbit ES-like cell was strongly positive;rabbit ES-like cells could become embryonic bodies-like structures in vitro, and embryonic bodies which stuck to plates could be differentiated into epithelial-like cells or scattered single cell;rabbit ES cells cluster were identical island-like image and grew coincidently by culture after routin freeze; karyotype analysis of the 5th passage rabbit ES cells was researched, and the karyotype normal rate was over 75%. The essay showed SD rat cardiomyocyte conditioned medium can be used to isolation and culture of rabbit ES-like cell.

关 键 词：兔 细胞培养 大鼠心肌细胞条件培养基 类胚胎干细胞 

分 类 号：Q813.11[生物学—生物工程]