兰尼碱受体mRNA在哮喘豚鼠气道平滑肌细胞中表达的改变  

Changes of ryanodine receptors mRNA expressions in airway smooth muscle cells of asthmatic guinea pigs

作　　者：封瑞[1] 滕赞[1] 赵美眯[1] 郝丽英[1] 李智[1] 

机构地区：[1]中国医科大学药学院天然药物研究室,辽宁沈阳110001

出　　处：《中国药理学与毒理学杂志》2007年第2期137-139,共3页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金资助项目(30470765)~~

摘　　要：目的明确兰尼碱受体(RyR)与哮喘发病的关系,为哮喘的防治提供一条新的途径。方法以酶消化法分离培养正常与哮喘豚鼠气道平滑肌细胞(ASMCs),应用RT-PCR方法测定每组细胞RyR各亚型RyR1,RyR2和RyR3的mRNA表达。结果正常与哮喘豚鼠ASMCs中,RyR1和RyR2的mRNA均见表达,未见RyR3mRNA的表达,并以表达RyR2mRNA为主。哮喘时,RyR1和RyR2的mRNA相对吸光度值分别为0.43±0.05和1.5±0.6,明显高于正常组RyR1mRNA的吸光度值0.34±0.03(n=6,P<0.01)。结论RyR1mRNA表达上调可能与哮喘发病相关。AIM To investigate the roles of ryanodine receptors （RyR） in asthma, and try to provide a new target for asthma treatment. METHODS Airway smooth muscle cells （ASMCs） were obtained by enzyme separation method from normal and asthmatic guinea pigs. The expressions of RyR1, RyR2 and RyR3 mRNA were determined by RT-PCR. RE- SULTS Both in normal and asthmatic guinea pigs, there were mRNA expressions of RyR1 and RyR2 in ASMCs, but no RyR3 mRNA. In asthma group, the expressions of RyR1 mRNA and RyR2 mRNA were 0.43 ± 0.05 and 1.5± 0.6, respectively. RyR1 mRNA of asthmatic group expressed significantly higher than that of normal group （0.43± 0.05 vs 0.34±0.03 ）. CON- CLUSION The up-regulation of RyR1 mRNA might be closely related to asthma.

关 键 词：哮喘 受体 兰尼碱 肌 平滑 气道 

分 类 号：R562.25[医药卫生—呼吸系统]