IL-2体外诱导健康人外周血T细胞TCRβ链CDR3谱系漂移的研究  

Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells cultured with interleukin-2

作　　者：常红[1] 罗薇[2] 马骊[2] 周明乾[2] 温茜[2] 吴远彬[1] 黄宇贤[1] 郭坤元[1] 

机构地区：[1]南方医科大学珠江医院血液科,广东广州510282 [2]南方医科大学生物技术学院分子免疫学研究所,广东广州510515

出　　处：《南方医科大学学报》2007年第4期433-435,438,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30471636)~~

摘　　要：目的研究IL-2对体外培养的T细胞TCRβ链CDR3谱系漂移的影响。方法分离健康志愿者外周血T细胞,加入IL-2,常规体外培养,乳酸脱氢酶释放法检测其非特异性杀伤活性;采用免疫谱型分析技术,分析其CDR3长度以判断T细胞的克隆性。结果3例健康志愿者外周血T细胞对鼻咽癌细胞系CNE2没有杀伤作用;PBMC的TCRVβCDR3谱型均呈高斯分布,经IL-2体外培养后部分家族出现不同的优势表达。结论IL-2对体外培养的T细胞TCRβ链CDR3谱系漂移有一定影响,对其非特异性杀伤无影响。Objective To analyze the drift of the complementarity-determining region 3 （CDR3） ofT cell receptor beta （TCRβ） chain variable region in T cells of healthy volunteers cultured with intedeukin-2 （IL-2）. Methods T cells were isolated from the peripheral blood and cultured in vitro in the presence of IL-2. The non-specific killing effect of the cells was analyzed by LDH releasing assay, and the distribution of TCRβ chain CDR3 in healthy volunteers by immunoscope spectratyping method to evaluate the clonality of the T cells. Results The results showed Gaussian distribution of TCR Vβ gene CDR3 in healthy volunteers. The T cell cultured with IL-2, however, displayed some anomalous and oligoclonal expansion in different TCR Vβ families without killing effect against nasophargngal carcinoma cell line CNE2. Conclusion IL-2 may affect TCRβ chain CDR3 distribution in T cells cultured in vitro.

关 键 词：IL-2 T细胞受体 互补决定区3 免疫扫描谱型分析 

分 类 号：R392.1[医药卫生—免疫学]