骨髓干细胞在大鼠移植肝中的诱导分化  

Induced differentiation of bone marrow stem cells in transplanted rat liver

作　　者：孔凡东[1] 潘明新[1] 王海澜[1] 单毓强[1] 高毅[1] 

机构地区：[1]南方医科大学珠江医院普外科,广东广州510282

出　　处：《南方医科大学学报》2007年第4期468-470,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金重点项目(037055)~~

摘　　要：目的研究骨髓干细胞在大鼠移植肝中的诱导分化情况及对大鼠长期存活的影响。方法雌性受体大鼠随机分3组:空白对照组(A组)、D-hanks液组(B组)、骨髓干细胞组(C组)。观察大鼠的中位生存时间、肝组织病理变化,基因Sry原位杂交和甲胎蛋白免疫组化双检测观察骨髓干细胞的分化情况。结果C组中位生存时间大于180d(P<0.05);C组无明显的急性排斥反应。基因Sry原位杂交阳性,并且表达甲胎蛋白。结论骨髓干细胞门静脉输注能减轻急性排斥反应,诱导大鼠肝移植术后长期存活;并能在移植肝的环境中诱导分化为肝细胞,表达甲胎蛋白,并逐渐替代移植肝本身的细胞。Objective To investigate the differentiation of bone marrow stem cells in transplanted livers and its impact on the long-term survival of rats with orthotopic liver transplantation. Methods Twenty-four female recipient rats with orthotopic liver transplantation were randomized into blank-control group, D-hanks solution group, bone marrow stem cells group with postoperative infusion of stem cells, and the pathological changes of the liver grafts and survival time of the rats were observed. The differentiation of the bone marrow stem cells were assessed 60 days after transplantation using in situ hybridization histochemistry for Sty gene and alpha-fetoprotein （AFP） immtmohistochemistry. Results In rats with postoperative infusion of bone marrow stem cells through the portal vein, the median long-term graft survival time exceeded 180 days, significantly longer than that in the other two groups （P〈0.05）, and no obvious evidence of acute rejection was observed with positive Sty expression and AFP expression. Conclusion Infusion of bone marrow stem cells through the portal vein following liver transplantation may alleviate acute graft rejection and promote long-term liver graft survival and AFP expression.

关 键 词：大鼠 肝移植 急性排斥反应 骨髓干细胞 

分 类 号：R657.3[医药卫生—外科学]