肿瘤坏死因子α对人脐静脉内皮样细胞增殖及syndecan-4蛋白表达的影响  

Tumor necrosis factor-alpha regulates the proliferation and syndecan-4 expression of human umbilical vein endothelial-like cells cultured in vitro

作　　者：张彬[1] 欧阳平[1] 陈烨[1] 赖文岩[1] 谢晋国[1] 许顶立[1] 

机构地区：[1]南方医科大学南方医院心血管内科,广东广州510515

出　　处：《南方医科大学学报》2007年第4期496-498,共3页Journal of Southern Medical University

基　　金：广州市科技局应用基础研究项目(2002Z3-E4131)

摘　　要：目的观察肿瘤坏死因子α(TNF-α)对体外培养的人脐静脉内皮样细胞株ECV304增殖及syndecan-4蛋白表达的影响。方法体外培养人脐静脉内皮样细胞株ECV304,分别应用1、10、20、100ng/ml的TNF-α作用24h及36h并设立对照组进行比较,采用MTS/PES法确定人脐静脉内皮样细胞的增殖状态。利用Westernblotting蛋白免疫印迹法测定细胞syndecan-4蛋白的表达情况。结果24h各组细胞增殖率分别为对照组(1.956±0.214),TNF-α100ng/ml组(2.154±0.250),TNF-α20ng/ml组(2.26±0.151),TNF-α10ng/ml组(2.118±0.205),TNF-α1ng/ml组(2.106±0.136)。统计分析显示低至1ng/ml的TNF-α仍能明显刺激人脐静脉内皮样细胞的增殖(P<0.05),其中以TNF-α20ng/ml组细胞增殖最显著P<0.05)。36h各剂量组的细胞增殖率均明显低于24h的细胞增殖率(P<0.05)。TNF-α对人脐静脉内皮样细胞的syndecan-4蛋白表达有显著的增强作用(P<0.05)。结论TNF-α对体外培养的人脐静脉内皮样细胞的增殖及syndecan-4蛋白表达均有明显的促进作用,但随着时间的改变,这种作用有所变化。Objective To investigate the effects of tumor necrosis factor-α （TNF-α） on syndecan-4 protein expression and proliferation of cultured human umbilical vein endothelial-like cells （HUVECs） in vitro. Methods HUVECs exposed to different concentrations of TNF-α（100, 20, 10, and 1 ng/ml） were cultured for 24 h and 36 h to observe their proliferation in comparison with the control group. The cell proliferation rate was determined by non-radioactive MTS/PES assay. The expression of syndecan-4 protein was evaluated by immunoblotting technique using anti-syndecan-4 antibody. Results The proliferation rate of the endothelial-like cells was 1.956±0.214 in the control group, and 2.154±0.250, 2.260±0.151, 2.118± 0.205 and 2.106±0.136 in TNF-α-treated groups corresponding to TNF-α concentrations of 100, 20, 10 and 1 ng/ml at 24 h, respectively. It was shown that TNF-α significantly stimulated cell proliferation at the concentration above 1 ng/ml （P〈0.05） as compared with the control group （P〈0.05）. The proliferation rate of the endothelial-like cell was 1.915±0.236 in the control group, and 2.067±0.328, 2.207 ±0.150, 2.052 ±0.126 and 2.051 ±0.180 in TNF-α-treated groups corresponding to TNF-αconcentrations of 100, 20, 10 and 1 ng/ml at 36 h, respectively. The expression of syndecan-4 protein was significantly enhanced by TNF-α. Conclusions TNF-α can stimulate HUVEC proliferation, and expression of syndean-4 may represent an additional component of the pro-inflammatory, growth-stimulating pathways that are activated in response to changes in the vascular wall.

关 键 词：syndecan-4蛋白 肿瘤坏死因子 人脐静脉内皮细胞 细胞增殖 

分 类 号：R541[医药卫生—心血管疾病]