胰高血糖素样肽-1类似物对糖尿病大鼠细胞免疫功能的影响  

Effects of GLP-1 analog on cellular immune function of diabetes mellitus rats

作　　者：赵海军[1] 张桂英[1] 徐琪[2] 刘娅[1] 于婷[1] 杨丽娜[1] 王洪莹[1] 谢丽波[1] 周静[2] 

机构地区：[1]吉林大学公共卫生学院营养与食品卫生教研室,吉林长春130021 [2]杭州中肽生化有限公司

出　　处：《中国老年学杂志》2007年第7期610-612,共3页Chinese Journal of Gerontology

基　　金：吉林省科技发展重点资助项目(20050413-4);2005年教育部博士点基金资助项目

摘　　要：目的探讨胰高血糖素样肽-1(GLP-1)类似物对实验性糖尿病的细胞免疫功能的影响。方法84只Wistar大鼠,采用高脂饲料饲养和注射40mg/kgSTZ方法建立实验性糖尿病动物模型。GLP-1类似物治疗2w后,采用酶化学方法检测空腹血糖;称量动物的体重和胸腺、脾脏的重量,计算胸腺指数和脾指数;通过MTT法观察淋巴细胞增殖,计算刺激指数(SI)。结果GLP-1类似物治疗2w后,糖尿病模型组和各治疗组与正常对照组和高脂饲料组相比较胸腺指数存在显著性差异(P<0.01);各组动物的脾指数之间没有显著性差异(P>0.05)。GLP-1类似物治疗2w后,各组动物之间相比较淋巴转化指数无显著性差异(P>0.05)。结论GLP-1类似物对糖尿病动物的细胞免疫功能没有明显的影响。Objective To explore effects of GLP-1 analog on cellular immune function of experimental diabetes mellitus （DM） rats. Methods 84 Wistar rats were used to establish experimental DM rats model by high fat forage （HFF）and injection of 40 mg/kg STZ. After 2 weeks of GLP-1 analog therapy, the level of fasting plasma glucose （FPG）was detected with enzyme-chemical method; the body weight and weight of thymus and spleen were measured for spleen and thymus index; Lymphocyte proliferation was observed with MTr for stimulation index （SI）. Results After 2 weeks of GLP-1 analog injection, thymus index of DM and all therapeutic group was lower than that in normal control group and HFF group （P 〈 0.01 ）, but there were no significant difference in spleen index among all groups （P 〉 0.05 ） ;there was no significant difference in SI among all groups （P 〉 0.05 ）. Conclusions GLP-1 analog has no significant effect on cellular immune function of DM rots.

关 键 词：胰高血糖素样肽-1 糖尿病 细胞免疫功能 淋巴细胞转化 

分 类 号：R587.1[医药卫生—内分泌]