RP-HPLC法测定齐多夫定肉豆蔻酸酯脂质体药物含量及包封率  

RP-HPLC determination of the content and entrapment efficiency of zidovudine myristrate liposomes

作　　者：金圣煊[1] 王艳芝[1] 胡会国[1] 邓意辉[1] 毕殿洲[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2007年第4期226-228,231,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(30371694)

摘　　要：目的建立齐多夫定前体药物一齐多夫定肉豆蔻酸酯脂质体含量及包封率的测定方法。方法采用RP-HPLC法。色谱柱为Diamonsil^TM ODSC18（4．6mm×200mm，5μm），流动相为甲醇-水（体积比为95：5），流速为1．0mL·min^-1，紫外检测波长为265nm；采用超速离心法分离齐多夫定肉豆蔻酸酯脂质体中的游离药物。结果在上述色谱条件下辅料和试剂对药物的测定无干扰，齐多夫定肉豆蔻酸酯浓度在1．05×100～6．28×100mol·L^-1内与峰面积的线性关系良好（r=0．9999），回收率在99．4％～102．0％之间，日内及日间RSD均小于2％（n=5）。结论RP-HPLC法可用于齐多夫定肉豆蔻酸酯脂质体含量及包封率的测定。Objective To establish a RP- HPLC method for content and entrapment efficiency determination of zidovudine myristrate liposomes. Methods The separation was performed with a Diamonsil TM ODS Cls column （4.6 mm × 200 mm, 5μm） using methanol - water （ V ： V = 95 ： 5） as the mobile phase and detected at 265 nm. The flow rate was 1 mL· min^-1 and 20μL sample solution was injected for each time. Results The calibration curve was linear within the range of 1.05 × 10^- 5 - 6.28 × 10^-5 mol·L^-1（ r = 0. 999 9）, the intra - day RSD and inter - day RSD were less than 2 %, and the average recovery was between 99.38 % - 101.95 % （n = 5）. Conclusions The method is simple, accurate, sensitive and applicable for determination of content and entrapment efficiency of zidovudine myristrate liposomes.

关 键 词：齐多夫定 齐多夫定肉豆蔻酸酯 脂质体 包封率 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]