全身照射引起间充质干/祖细胞池缩小及成骨潜能改变  

Reduction of Bone Marrow Mesenchymal Stem/Progenitor Cells Pool and Alteration of Their Osteogenesis Potential Caused by Total Body Irradiation

作　　者：马杰[1] 陈斌[1] 王宏兰[1] 李静[1] 史明霞[1] 李炳宗[1] 胡建立[1] 赵春华[1] 

机构地区：[1]中国医学科学院基础医学研究所,中国协和医科大学基础医学院组织工程研究中心,北京100005

出　　处：《中国实验血液学杂志》2007年第2期313-318,共6页Journal of Experimental Hematology

基　　金：国家高技术研究发展计划863资助项目(编号2002AA205061);国家杰出青年基金资助项目(编号30125018)

摘　　要：为了研究辐射对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSC)数量及成骨分化潜能的影响,探讨BMMSC在辐射损伤中的反应及其与照射后造血和骨骼系统长期损伤的关系,用全身照射(total body irradiation,TBI)小鼠模型观察TBI前后不同时间点小鼠骨髓纤维母细胞集落形成单位(colony-forming unit-fibroblast,CFU-F)的改变及BMMSC细胞周期分布情况;用Von Kossa染色法测定钙盐沉积,实时定量RT-PCR检测成骨分化相关基因及成骨转录共刺激因子TAZ(transcriptional coactivator with PDZ-binding motif)的表达变化。结果显示:TBI后骨髓CFU-F数量明显减少;TBI后3天较多的BMMSC进入细胞周期并且其成骨潜能明显增强,随后细胞周期分布恢复正常,但成骨潜能明显降低,同时伴有TAZ表达改变。结论:TBI能导致小鼠骨髓间充质干/祖细胞池的显著缩小并改变BMMSC的成骨分化潜能,TAZ表达的变化可能在其成骨潜能的改变中发挥一定作用。To investigate the effect of irradiation on the quantity and osteogenesis potential of BMMSCs and to explore the response of them in the irradiation stress and its contribution to long-term effects of radiation-induced bone and hematologic injury, a total body irradiation （TBI） murine model was adopted. The number of CFU-F and cell cycle profile of BMMSCs were analyzed at different time points before and after TBI. Osteogenic differentiation was evaluated by Von Kossa staining, expressions of osteogenesis-related genes and transcriptional coactivator with PDZ-binding motif （TAZ） were detected by real-time RT-PCR. The results showed that the number of CFU-F decreased greatly at day 28 after TBI. At day 3 after TBI, more cells entered cell cycle and the osteogenesis potential was greatly enhanced followed by recovery of cell cycle distribution and signifiacant defect in osteoblast differentiation respectively, meanwhile the expression of TAZ was changed. It is concluded that TBI results in the reduction of bone marrow mesenchymal stem/ progenitor cell pool and alters the osteogenesis potential of BMMSCs, which is related to the change of TAZ expression.

关 键 词：全身照射 骨髓间充质干细胞 成骨潜能 TAZ 

分 类 号：Q691[生物学—生物物理学] R329.28[医药卫生—人体解剖和组织胚胎学]