拮抗TGF-β_1可抑制胚胎干细胞来源BL-CFC的产生和分化  

Reduction of Blast-Colony Forming Cell Development and Bi-potential Commitment by Neutralizing TGF-β_1 In Vitro

作　　者：要晖宇[1] 刘兵[1] 王晓燕[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所分子生物学研究室,北京100850

出　　处：《中国实验血液学杂志》2007年第2期324-327,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金面上项目(编号30300181);国家973计划项目(编号2005CB522705)

摘　　要：胚胎干细胞来源的BL-CFC体外可分化产生造血和内皮细胞。为了研究TGF-β1对BL-CFC产生和分化的调控作用,在拟胚体培养阶段施加TGF-β1和TGF-β1中和抗体,观察不同阶段TGF-β1对BL-CFC的数目及其定向内皮和造血细胞分化能力的影响。结果表明:在拟胚体培养阶段拮抗TGF-β1的作用可显著降低BL-CFC的数量(P<0.01);与之吻合的,中和TGF-β1后拟胚体中Flk-1+细胞比例降低。此外,在拟胚体培养阶段加入TGF-β1可显著增强BL-CFC的造血和内皮细胞分化能力,而中和TGF-β1后分化能力降低。结论:TGF-β1对BL-CFC的产生和分化发挥正向调节作用。To study the regulation of TGF-β1 on the development of hemangioblast, embryonic stem cell-derived blast forming cells （BL-CFC） were used as the model of hemangioblast in vitro. TGF-β1 or anti-TGF-β1 neutralization antibody was added in the medium of embryoid body （EB） generation for observating influence of TGF-β1 addition in different culture stages on number of BL-CFC and differentiation of BL-CFC to endothelial and hematopoietic cells. The results showed that antagonizing TGF-β1 in the course of EB growth could significantly reduce the number of BL-CFC （P 〈0. 01 ）, and the frequency of Flk-1^＋ cells was also decreased consistently. Furthermore, the BL-CFC derived from EB pretreated with TGF-β1 demonstrated remarkably elevated hematopoietic and endothelial potential, whereas such bipotential was impaired in the group with neutralizing antibody. It is concluded that TGF-β1, a conventional negative regulator in hematopoiesis and angiogenesis exert positive effects on the development and differentiation capacities of BLCFC in vitro.

关 键 词：TGF-Β1 BL-CFC 胚胎干细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R331.2[医药卫生—基础医学]