间日疟原虫重组蛋白Pvs25和Pvs28免疫小鼠应答机制观察  

Mechanism of murine immune response to Plasmodium vivax Pvs25 and Pvs28 recombinant proteins

作　　者：李冬梅[1] 刘军[1] 刘英杰[2] 郑丽[1] 冯辉[1] 陈光[1] 曹雅明[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳110001 [2]中国医科大学基础学院病原生物学教研室,沈阳110001

出　　处：《中国人兽共患病学报》2007年第4期348-350,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目(No.30371347)

摘　　要：目的观察间日疟传播阻断疫苗候选抗原Pvs25和Pvs28的免疫应答机制。方法分别用与弗氏佐剂乳化后的Pvs25和Pvs28重组蛋白皮下免疫DBA/2小鼠;采用ELISA分别对各组小鼠脾细胞培养上清的IFN-γ、IL-4、IL-10动态检测;以ELISPOT方法检测小鼠脾脏IFN-γ+和IL-4+细胞数量。结果经重组蛋白免疫的两组小鼠脾细胞培养上清IFN-γ的含量于初次免疫后第14d升高,与对照组相比差异显著(P<0.01),但随后降至对照组水平;IL-4和IL-10均在初次免疫后第28d出现有意义的升高(P<0.01),后升高更为明显,分别直至免疫后期70d、56d出现下降,但直至112d仍明显高于免疫前的水平(P<0.01)。初次免疫后第70d?98d小鼠脾脏IFN-γ+和IL-4+细胞数量,与对照组相比IFN-γ+无明显变化,而IL-4+则明显增多(P<0.01)。结论Pvs25和Pvs28重组蛋白可诱导小鼠产生以Th2反应为主的免疫应答。The mechanism of murine immune response to Pvs25 and Pvs28 recombinant protein （ rPvs25 and rPvs28 ）, the Plasmodium vivax malaria transmission blocking vaccine candidates, was investigated. Mice were injected subcutaneouly with rPvs25 or rPvs28 emulsified in Freund＇s adjuvant. Level of IFN-γ, IL-10 and IL-4 in spleen cell culture supernatants derived from mice at different times before or after vaccination was assayed by ELISA kits. Numbers of IL-4^＋ and IFN-γ^＋ cells in murine spleen lymphocytes were detected by enzyme-linked immunospot assay （ ELISPOT） on day 70, day 98 after first vaccination. It was found that level of IFN-γ elevated sharply at day 14 （P〈0.01} and then dropped to the level of control group. Levels of IL-4 and IL-10 increased from day 28 after first vaccination and got further higher henceforth. Despite of its later decrease, the levels kept higher till day 112 compared with the control （ P〈0.01）. Number of IL-4^＋ ceils from mice spleen elevated on day 70 and day 98 （ P〈0.01）, while that of IFN-γ^＋ cells didn＇t change, compared with the control. It is suggested that rPvs25 and rPvs28 can induce murine immune response, predominantly the Th2 response.

关 键 词：间日疟原虫 传播阻断疫苗 PVS25 Pvs28 细胞因子 

分 类 号：R382[医药卫生—医学寄生虫学]