抗鼠疟免疫核糖核酸治疗约氏疟原虫感染的疗效观察  

Observations on therapeutic effect of iRNA against murine malria on malaria caused by Plasmodium yoelii

作　　者：刘英杰[1] 杨志蕃[2] 阎建忠[3] 陈光[3] 崔洋[3] 

机构地区：[1]中国医科大学病原生物学教研室,沈阳110001 [2]沈阳军区总医院,沈阳110015 [3]中国医科大学免疫学教研室,沈阳110001

出　　处：《中国人兽共患病学报》2007年第4期376-377,397,共3页Chinese Journal of Zoonoses

摘　　要：目的观察抗鼠疟免疫核糖核酸的疗效。方法以约氏疟原虫腹腔感染DBA／2和BALB／c小鼠，并用抗鼠疟免疫核糖核酸对其进行治疗。薄血膜染色，计数红细胞感染率；用ELISA试剂盒和Griess实验分别检测DBA／2小鼠牌细胞培养上清中IFN-γ和NO含量。结果注射正常核酸的BALB／c小鼠于感染后6～7d全部死亡，与其相比，注射免疫核糖核酸BALB／C小鼠虫体血症蜂值出现的时间被推迟2d，并且有20％的小鼠被治愈；注射免疫核糖核酸DBA／2小鼠牌细胞分泌的IFN-γ水平比对照组略有升高，但NO水平升高显著，其红内期原虫的清除时间也比对照组提前了4天。结论抗鼠疟免疫核糖核酸可使抵抗宿主的巨噬细胞活化状态得以进一步强化，其对约氏疟原虫感染所致的鼠疟具有一定疗效。To ascertain therapeutic effect of iRNA on murine malaria, BALB/c and DBA/2 mice were infected intraperitoneally with erythroey＇tes parasitized with P. yoelii 17XL （lethal）, then treated by injection of iRNA. The levels of parasitemia were observed during infection by Giemsa stain of thin blood smear, and the levels of IFN-γ and NO in culture supematants of splenocytes from DBA/ 2 mice were also measured by ELISA kits and Griess reaction, respectively. It was found that all BALB/c mice injected nRNA died from the 6^th to 7^th day post-infection, the occurrence of parasitemia peak in BALB/c mice injected iRNA was later 2 day than that of control group, and 20% mice were cured. The levels of IFN-7 in culture supernatants of splenocytes from DBA/2 mice injected iRNA were higher than that of control mice, however, their NO levels increased obviously, and the elimination time of parasitemia in these rniee injected iRMA was earlier 4 day than control mice injected nRNA. These results suggest that activated state of macrophages in reaistant mice can be farther intensified by injection of iRNA, and anti-malaria iRNA has mildly therapeutic effect on murine malaria.

关 键 词：约氏疟原虫 免疫核糖核酸 疗效观察 

分 类 号：R531.305[医药卫生—内科学]