复合rhVEGF+rhBMP-2的钙磷骨水泥促进犬异体脱蛋白骨关节移植的成骨研究  

Experimental study on ossification of deproteinized osteoarticular allograft with calcium phosphate cement containing recombinant human vascular endothelial growth factor and bone morphogenetic protein-2 in dogs

作　　者：瞿向阳[1] 蒋电明[1] 安洪[1] 曹本珍[1] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《重庆医科大学学报》2007年第5期484-488,共5页Journal of Chongqing Medical University

基　　金：重庆市科技攻关项目:渝科发计字200138号。

摘　　要：目的:探讨钙磷骨水泥(calcium phosphate cement,CPC)复合重组人血管内皮细胞生长因子(Recombinant human vas- cular endothelial growth factor,rhVEGF)+重组人骨形态发生蛋白-2(Recombinant human bone morphogenetic protein-2,rhBMP-2)与异体脱蛋白骨(Deproteinized bone,DPB)关节复合移植修复骨关节缺损的能力。方法:成年杂交犬36只,距关节面30mm切除右侧股骨下端制成半关节缺损模型,随机分成三组:A组:CPC/rhVEGF/rhBMP-2/DPB关节移植组;B组:rhVEGF/rhBMP-2/DPB关节移植组;C组:单纯的DPB关节移植组。于术后4、8、12、16周行大体形态学观察及X线检查、组织学检查并做I型胶原免疫组织化学分析、透射电镜检查和生物力学测试,检测骨修复情况。结果:A组在大体形态学观察、X线片、组织学、电镜观察和生物力学测试,其成骨能力和质量均优于B、c两组,I型胶原免疫组化图像分析和生物力学测试结果均优于B、C两组,差异有统计学意义(P<0.01),B、C组间在各时间点差异无统计学意义(P>0.05)。结论:CPC/rh- VEGF/rh-BMP-2可促进异体脱蛋白骨关节移植的成骨能力,可加速移植体与受体骨愈合。Objective：To explore the ability of osteo-articular allograft of variant deproteinized bone combining calcium phosphate cement with recombinant human vascular endothelial growth factor and bone morphogenetic protein-2 in repairing osteo-articular defect. Methods：36 adult mixtus dogs were constructed as semi-articular defect models through resecting 30mm right femur inferior extremity near articular facet,and they were randomly divided into 3 groups.In group A,material CPC/rh VEGF/rh BMP-2/DBP was transplanted into the semi-articular defect. In group B,material rhVEGF/rhBMP-2 /DBP was transplanted there. In group C,simple material DPB was transplanted there.At 4,8,12 and 16 weeks after operation,the effects were assessed by macro-morphology observation,X-ray examination,histologic examination as well as collagen type I immunohistochemistry analysis ,transmission electron microscope examination and vitodynamic tests in order to observe bone reparation mattero Results：The new bone formation observed by vitodynamic tests,macro- morphology observation,X-ray examination,histologic examination and transmission electron microscope examination in group A was significantly superior to that in group B and C.The results of collagen type I immunohistochemistry analysis and vitodynamic tests were significantly superior to that in group B and C（P〈0.01）. However there was not statistically significant difference between group B and C（P〉0.05）.Conclusion：The materials CPC/ rh VEGF/rh BMP-2 can promote the new bone formation, achieve bone healing and make them become autologous tissue at last.

关 键 词：钙磷骨水泥 脱蛋白骨 血管内皮细胞生长因子 骨形态发生蛋白 骨关节移植 

分 类 号：Q813[生物学—生物工程] R687.34[医药卫生—骨科学]