异丙酚对大鼠局灶性脑缺血再灌注损伤细胞凋亡的影响  

Effects of propofol on cell apoptosis in ischemia-reperfusion injury in rat brain

作　　者：刘鹏斌[1] 蔡英敏[1] 宋金鑫[1] 

机构地区：[1]西安交通大学医学院第二附属医院麻醉科,陕西西安710004

出　　处：《第四军医大学学报》2007年第8期682-684,共3页Journal of the Fourth Military Medical University

摘　　要：目的:研究异丙酚对大鼠局灶性脑缺血再灌注损伤中细胞凋亡的影响.方法:SD大鼠60只,体质量290～310g,随机分为假手术组、异丙酚组和对照组,每组20只.用改良Longa法制成鼠脑缺血/再灌注模型(缺血2h,再灌2h),光镜下观察细胞形态学变化,TUNEL法观察细胞凋亡变化.结果:光镜检查异丙酚组细胞肿胀坏死明显减轻,凋亡细胞及坏死细胞明显减少.再灌2h后对照组凋亡细胞比率、凋亡细胞阳性率、TUNEL阳性率均较假手术组高(P<0.01),异丙酚组凋亡细胞阳性率、TUNEL阳性率较对照组减少(P<0.05).结论:异丙酚能减轻大鼠局灶性脑缺血再灌注损伤,具有抑制神经细胞凋亡坏死的作用.AIM： To study the effects of propofol against cell apoptosis due to ischemia-reperfusion （I/R） injury in rat brain. METHODS： Sixty healthy male SD rats, weighing 290 -310 g were selected. Modified Longa model of fecal cerebral I/R injury was used （2 h isehemia and 2 h reperfusion）. These SD rats were randomly divided into 3 groups： sham operation group, I/R group and propofol pretreatment group. After operation, rats were killed and brains were removed for the detection of apoptotic neurons using TUN-EL staining combined with electron microscopic examination. RESULTS： The number of apoptotic neurons of propofol group was significantly less than that of I/R group and sham operation group（P 〈0. 01 ）. The TUNEL positive rate, and apoptosis rate were significantly lower in propofol group than those in I/R group （P 〈 0. 05 ）. CONCLUSION： Propofol pretreatment decreases neuronal apoptosis induced by I/R, which relieves the brain injury caused by I/R.

关 键 词：二异丙酚 大鼠 脑缺血 再灌注损伤 

分 类 号：R338.2[医药卫生—人体生理学]