主动载药法制备苦参碱脂质体的研究  

Preparation of Matrine Liposomes by Active Loading Method

作　　者：刘文雅[1] 刘汉清[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《时珍国医国药》2007年第4期908-909,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的考察脂质体不同制备方法对包封率的影响。方法采用被动载药和主动载药制备脂质体,用微柱离心-紫外分光光度法测定脂质体包封率。结果被动载药法制备脂质体包封率较低,主动载药制备脂质体包封率为84.38%。结论采用硫酸铵梯度法可制得包封率较高的苦参碱脂质体。Objective To study the influence of different preparation methods on the entrapment efficiency. Methods To prepare matrine liposomes by passive loading method and avtive loading method, the entrapment efficiencies were determined by minicolumn centrifuge - UV method. Results The entrapment efficiency with passive loading method was low, while up to 84.38% with active loading method. Conclusion The matrine liposomes with high entrapping efficiency can be prepared by ammonium sul- phate gradien method.

关 键 词：苦参碱 脂质体 主动载药 被动载药 

分 类 号：R283.1[医药卫生—中药学]