X射线全身照射诱导小鼠脾细胞LAMP-1表达的时程变化  

Change of time-course in expression of LAMP-1 on the splenocytes after WBI of mice with X rays

作　　者：徐瑞明[1] 陈光伟[2] 武宁[1] 刘永哲[1] 刘扬[1] 金顺子[1] 刘树铮[1] 

机构地区：[1]吉林大学公共卫生学院；卫生部放射生物学重点实验室,长春130021 [2]吉林省卫生厅卫生监督所

出　　处：《中华放射医学与防护杂志》2007年第2期121-124,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30100033）

摘　　要：目的观察X射线全身照射对小鼠脾细胞LAMP-1表达的影响。方法采用流式细胞术检测0．075和2Gy X照射后不同时间（0、2、4、8、16、24和48h）以及用Con A刺激4h后，脾细胞表面表达LAMP-1的阳性细胞数。结果脾细胞表面的LAMP-1在2Gy X射线全身照射后8、16和24h表达明显下降；0．075Gy X射线全身照射后2h显著升高，而48h又下降到低于对照的水平。0．075和2Gy X射线全身照射后再加用Con A（10μg／μl）刺激4h，LAMP-1的表达均增强；但是此时2Gy的效应比0．075Gy更明显。结论LAMP-1参与电离辐射作用下免疫信号的传导，高剂量X射线抑制它的表达，而低剂量X射线在早期对其表达有促进作用，与体内免疫细胞的活性密切相关。Con A促进免疫细胞表面LAMP-1的表达。Objective To study the effect of WBI with X rays on the expression of LAMP-1 in the splenocytes of mice. Methods The LAMP-1 positive cells from mouse splenocytes were detected with flow cytometry （FCM） at different times（0,2,4,8,16,24 and 48 h） after WBI with X-rays of 0.075 Gy and 2.0 Gy and after WBI added by Con A stimulation. Results In view of time-course effect, the LAMP-1 expression in the splenocytes was significantly inhibited at 8, 16, 24 h after WBI by 2.0 Gy X-rays while the LAMP-1 expression was markedly enhanced at 2 h and suppressed at 48 h after WBI by 0.075 Gy X-rays. Compared with control group ,the LAMP-1 expression in the splenocytes was significantly up-regulated with Con A stimulation（ 10 μg/μl） after WBI with 0.075 and 2.0 Gy X rays. With Con A added, the effect of 2.0 Gy X rays was greater than that of 0.075 Gy. Conclusions LAMP-1 plays a role in the transduction of immune signals induced by ionizing radiation and is closely correlated with the activity of immune ceils. The high dose irradiation inhibits the LAMP-1 expression while the low dose likely causes the opposite biological effect in the early time. Con A enhances the expression of LAMP-1 on the surface of immune ceils.

关 键 词：X射线 T细胞 NK细胞 LAMP-1 流式细胞术 

分 类 号：R686[医药卫生—骨科学]