基于神经细胞活性评价高功率微波照射安全性研究  

Effects of high power microwave irradiation on cellular activities of cultured primary neurons

作　　者：高艳[1] 秦树桐[1] 张成岗[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2007年第2期201-202,205,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家高技术研究发展专项经费,国家自然科学基金重大研究计划（90208017）,国家自然科学基金资助项目（30300358）

摘　　要：目的研究平均功率密度5mW／cm^2的高功率微波（HPM）对神经细胞活性的影响，为制定微波照射的安全标准提供参考数据。方法出生后12h内Wistar大鼠乳鼠皮层神经细胞常规培养7d后，进行HPM照射。采用细胞内ATP酶活性测定法，检测HPM对原代培养神经细胞总ATP酶、钙镁ATP酶和钠钾ATP酶活性的影响，并用MTT法检测照射后细胞琥珀酸脱氢酶（SDH）活性的变化，观察时间点分别为HPM照射后1和6h，1、3和7d。结果原代培养神经细胞经平均功率密度5mW／cm^2的HPM连续照射6min后，同一时间点内HPM照射组和对照组之间细胞SDH活性和各ATP酶活性均无显著差异。结论平均功率密度为5mW／cm^2的HPM照射对原代神经细胞的活性无显著影响，提示就神经细胞活性而言，该功率密度的HPM照射是安全的。Objective To evaluate the biological effects of high power microwave （HPM） irradiation on cellular activities of cultured primary neurons. Methods After the cerebral cortical neurons of newborn Wistar rats were cultured for 7 days, the cultured primary neurons were exposed to high microwave irradiation with an average power density of 5 mW/cm^2. The enzymatic activity of total ATPase, Ca^2＋, Mg^2＋-ATPase and Na^＋, K^＋-ATPase were measured at 1 and 6 h, 1, 3 and 7 d after irradiation. The enzymatic activities of succinate dehydrogenase （SDH） of the cultured primary neurons were also detected at the same time by the MTT method to reveal the activity of the neurons. Results The enzymatic activities of ATPase and SDH of neurons treated with microwave exposure with the average power density of 5 mW/cm^2 were not significantly changed. Conclusions The HPM exposure to neurons with an average power density of 5 mW/cm^2 is biologically safety to cultured primary neurons.

关 键 词：高功率微波 微波照射 神经细胞 ATP酶 细胞活性 

分 类 号：R686[医药卫生—骨科学]