哮喘患儿转录因子T-bet/GATA-3 mRNA表达及其与杀伤性T淋巴细胞平衡间的关系  

Expression of transcription factors T-bet/GATA-3 mRNA and its effect on Tc1/Tc2 balance in asthmatic children

作　　者：檀卫平[1] 麦贤弟[1] 吴葆菁[1] 李晓圆[1] 李静[1] 魏菁[1] 黄花荣[1] 黄绍良[1] 

机构地区：[1]中山大学附属第二医院儿科,广州510120

出　　处：《中华儿科杂志》2007年第4期284-287,共4页Chinese Journal of Pediatrics

基　　金：广东省科委自然科学基金资助(031668A)

摘　　要：目的研究转录因子 T-bet/GATA-3在小儿哮喘发病以及杀伤性 T 淋巴细胞 Tc1/Tc2失衡中的作用。方法通过半定量 RT-PCR 检测38例哮喘患儿(哮喘发作组20例,缓解组18例)及20例正常对照组儿童外周血单个核细胞(PBMC)中 T-bet、GATA-3mRNA 表达情况,同时采用三色荧光流式细胞仪检测哮喘患儿的 Tc1、Tc2细胞数量,并对 T-bet、GATA-3mRNA 表达水平与 Tc1、Tc2细胞比率进行相关分析,了解 T-bet/GATA-3对哮喘患儿的 Tc 细胞分化的调节作用。结果 (1)哮喘患儿外周血淋巴细胞转录因子 T-bet mRNA 表达水平低于正常对照组(0.28±0.03),其中哮喘发作组(0.14±0.04)显著低于哮喘缓解组(0.21±0.03)(P<0.05);转录因子 GATA-3 mRNA 表达水平高于正常对照组(0.37±0.04),其中哮喘发作组(0.49±0.09)高于哮喘缓解组(0.44±0.08)(P<0.05)。(2)哮喘患儿外周血淋巴细胞亚群 Tc1百分率低于正常对照组(31.2±3.8),其中哮喘发作组(6.6±2.4)又低于哮喘缓解组(14.2±4.3)(P<0.05);Tc2细胞百分率高于正常对照组(3.5±1.1),其中哮喘发作组(10.0±4.2)又高于哮喘缓解组(5.4±2.2)(P<0.05)。(3)哮喘患儿 T-betmRNA 表达量与 Tc1细胞比率呈正相关(r=0.704),与 Tc2细胞比率呈负相关(r=-0.629);GATA-3mRNA 表达量与 Tc1细胞比率呈负相关(r=-0.612),与 Tc2细胞比率呈正相关(r=0.673);T-bet/GATA-3 mRNA 比值与 Tc1细胞比率呈正相关(r=0.731),与 Tc2细胞比率呈负相关(r=-0.642),与 Tc1/Tc2比值呈正相关(r=0.773)。结论哮喘患儿 T-bet/GATA-3 mRNA 表达水平失调,与哮喘患儿的 T 细胞呈现 Tc2优势分化密切相关。Objective In contrast to CD4^＋ helper T-lymphocytes （TH ）, little is known about the transcriptional regulation of CD8^＋ cytotoxic T-lymphocytes （Tc） and its role in the pathogenesis of asthma is unclear. This study was conducted to investigate the effect of T-bet and GATA-3 mRNA expression on profiles of type 1 and type 2 cytotoxic T lymphocytes in asthmatic children. Method Totally 38 asthmatic children,including acute attack group composed of 20 cases （age 3 - 13 years, mean 6. 2 ±2. 9）, remission group with 18 cases （age 3 - 12 years, mean 6. 1 ±2. 5） and 20 healthy control children （age 3 - 12, 6. 9 ± 2.7） were recruited in this study from Sep. 2005 to Mar. 2006. The mRNA expression of T-bet and GATA-3 in the peripheral blood mononuclear cells were detected by using semiquantitative PCR and Tcl, Tc2 cell numbers by flow cytometry analysis system. Result T-bet mRNA in asthmatic children was lower than that in control group and lower in attack stage than in remission stage （0. 14 ±0.04,0.21 ±0.03,0.28 ± 0. 03, P 〈 0. 05 ）. In contrast, GATA-3 mRNA was higher in asthmatic children than in control group and higher in attack stage than in remission stage （0.49 ± 0. 09, 0. 44 ± 0. 08, 0. 37 ± 0.04, P 〈 0.05 ）. It was shown that Tcl percentage was lower in asthmatic children than those of control group and lower in attack stage than those of remission stage （6.6 ± 2.4, 14. 2± 4. 3, 31.2 ± 3.8, P 〈 0. 05 ）. Tc2 percentage in asthmatic children was higher than that of control group and higher in attack stage than that of remission stage （ 10. 0 ± 4. 2, 5.4 ± 2.2, 3.5 ±1.1, P 〈 0.05 ）. Spearman correlation analysis revealed that T-bet mRNA was positively correlated with Tc1 percentage （ r = 0. 704 ） and negatively correlated with Tc2 percentage （ r = -0. 629）. GATA3 mRNA was negatively correlated with Tcl percentage （r = -0. 612） and positively correlated with Tc2 percentage （r =0. 673）. The T-bet/GATA-3 mRNA ratio was positively correlate

关 键 词：哮喘 DNA结合蛋白类 反式激活因子类 T淋巴细胞 细胞毒性 

分 类 号：R725.6[医药卫生—儿科]