淋球菌MtrC融合蛋白单克隆抗体杂交瘤细胞株的建立和特性鉴定  

Establishment and characterization of mice hybridoma cell line secreting monoclonal antibody against MtrC of Neisseria gonorrhoeae

作　　者：林能兴[1] 陈宏翔[1] 黄长征[1] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤性病科,武汉430022

出　　处：《中国麻风皮肤病杂志》2007年第4期271-273,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金资助项目(编号:30371293)

摘　　要：目的:建立针对淋球菌MtrC膜蛋白的单克隆杂交瘤细胞株,制备相应的单克隆抗体(单抗),用于淋球菌耐药机制的研究和淋球菌耐药的临床检测。方法:用重组MtrC抗原免疫6周龄Balb/c小鼠,按常规淋巴细胞杂交瘤技术制备单抗。应用间接ELISA法和Western印迹法分别测定单抗亚类及其特异性。结果:建立了2株小鼠抗MtrC单抗杂交瘤细胞2B3和4F7,染色体数在95～103之间,能稳定分泌抗MtrC单抗,单抗类型为IgG1,腹水效价为1:105和1:106。结论:本研究建立的单抗在检测淋球菌MtrC膜蛋白时有高度特异性和敏感性。Objective： To develop and identify the monoclonal antibody against recombinant MtrC antigen, in order to provide a rational research basis for the study on quinolone resistance mechanism and clinical detection. Methods： Recombinant MtrC protein was used as antigen to immune Balb/c mice. Manoclonal antibodies against MtrC were prepared by normal hybridoma technology. The specificity and sensitivity of anti - MtrC antibodies （McAbs） were calculated according to the results of ELISA and Western blot methods, Results： Two cell lines of hybridoma, with chromosome numbers of 95 - 103 and constantly secreting McAbs, were obtained and named as 2B3 and 4F7, which produced specific McAbs. McAbs were IgG1 subclass and the liters of ascitic fluid were 1 ：10s and 1 ： 10^6 respectively. Conclusion ： The established McAbs in this study to detect MtrC protein of N. gonorrhoeae possesses high specificity and sensitivity.

关 键 词：单克隆抗体 淋球菌 杂交瘤细胞株 耐药机制 

分 类 号：R759.2[医药卫生—皮肤病学与性病学]