Effect of Gardenia extract ZG on the adsorption quantity of herpes simplex virus type 1 (HSV-1)  

作　　者：SHAN SHAN GUO YI ZHONG WANG XIU KUN WANG YING JIE GAO YA HONG JIN DE FENG LI GUI DONG YUAN YI ZHANG GUI ZHEN ZONG YE ZHAO XIAO LAN CUI 

机构地区：[1]Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing, P. R.China [2]Aerospace Central Hospital, Beijing, P. R. China

出　　处：《Journal of Microbiology and Immunology》2006年第4期272-277,共6页中华微生物学和免疫学（英文版）

基　　金：This subject was supported by the grants from the National Natural Science Foundation of China （ No. 30371754）.

摘　　要：To observe the effect of Gardenia extract ZG on the adsorption quantity of herpes simplex virus type 1 （HSV-1） so as to explore the mechanism of its antiviral activity, fluorescein isothiocyanate （FITC） was used as the fluorescent probe to label viruses and heparin sodium was used as control. Meanwhile, the effect of Gardenia extract ZG on the adsorption quantity on the surface of Hep-2 cells was determined by flow cytometry. It was demonstrated that adsorption of HSV-1 on the surface of Hep-2 cells exhibited the character of saturation and specificity and heparin sodium could prevent attachment of viruses on these ceils. These results are in accord with those reported previously. It was also proved that the manner of drug-use prior to adsorption or simultaneous use of drug and adsorption was better than adsorption prior to drug-use, and the inhibition rates of the former and latter manner were 84.76% and 82.92% respectively. Three manners of drug-use with Gardenia extract ZG were all effective to reduce the adsorption quantity of viruses, especially the manner of simultaneous use of drug and adsorption with an adsorption inhibition rate of 68.46%. From the above observation, it is apparent that the mechanism of anti-viral activity of Gardenia extract ZG may be via several steps involved in the HSV-1 adsorption.To observe the effect of Gardenia extract ZG on the adsorption quantity of herpes simplex virus type 1 (HSV-1) so as to explore the mechanism of its antiviral activity, fluorescein isothiocyanate (FITC) was used as the fluorescent probe to label viruses and heparin sodium was used as control. Meanwhile , the effect of Gardenia extract ZG on the adsorption quantity on the surface of Hep-2 cells was determined by flow cytometry. It was demonstrated that adsorption of HSV-1 on the surface of Hep-2 cells exhibited the character of saturation and specificity and heparin sodium could prevent attachment of viruses on these cells. These results are in accord with those reported previously. It was also proved that the manner of drug-use prior to adsorption or simultaneous use of drug and adsorption was better than adsorption prior to drug-use, and the inhibition rates of the former and latter manner were 84. 76% and 82.92% respectively. Three manners of drug-use with Gardenia extract ZG were all effective to reduce the adsorption quantity of viruses, especially the manner of simultaneous use of drug and adsorption with an adsorption inhibition rate of 68.46% . From the above observation, it is apparent that the mechanism of anti-viral activity of Gardenia extract ZG may be via several steps involved in the HSV-1 adsorption.

关 键 词：Gardenia extract ZG Herpes simplex virus type 1 （HSV-1） Adsorption quantity Flow cytometry （FCM） Fluorescein isothiocyanate （FITC） 

分 类 号：R275.921.1[医药卫生—中西医结合] R289.5[医药卫生—中医皮科]