检测临床分离的产金属β-内酰胺酶铜绿假单胞菌的基因型  

Detection and analysis of genotypes of pseudanosa aerguinosa producing metallo-β-lactamase in clinical isolates

作　　者：曹敬荣[1] 沈定霞[1] 罗燕萍[1] 白立彦[1] 张文利 周光[1] 

机构地区：[1]解放军总医院微生物科,北京100853 [2]乌鲁木齐市第一人民医院,新疆830002

出　　处：《军医进修学院学报》2007年第2期132-134,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的:研究铜绿假单胞菌中金属β-内酰胺酶的的基因型及基因的转移机制,为监控产酶菌株的流行及指导临床合理选择抗生素提供依据。方法:E-test筛选对亚胺培南或头孢他啶耐药的铜绿假单胞菌中的产金属β-内酰胺酶菌株,PCR扩增耐药基因imp、vim、spm和gim。接合试验检测质粒型耐药基因。结果:75株耐亚胺培南和/或头孢他啶的铜绿假单胞菌中21株E-test阳性,PCR检测imp基因阳性菌株11株,vim基因阳性菌株3株,spm基因可疑阳性菌株4株,3株经E-test试验阳性而用特异引物PCR扩增金属酶基因为阴性。接合试验未获得接合子。结论:我院和新疆地区临床分离的铜绿假单胞菌所产金属β-内酰胺酶基因型以IMP型为主。Objective：To study genetypes of metallo-β-lactamase in pseudomonas aeruginosa and analyze the transfer mechanism of resistance genes. Methods： Metallo-β-lactamase positive strains were screened from imipenem-resistant or ceftazidime-resistant pseudomonas aeruginosa isolates by E test strips of imipenem with and without EDTA. PCR was used to amplify the resistant genes of imp, vim, spm and g/m. Resistance genes transmitted by plasmids was determined by conjugation. Results： 21 strains of Pseudomonas aeruginosa were shown to produce metallo-β-1actamase by E test, and they were used as templates to amplify imp, vim, spm and gim genes. Among them,specific bands of imp,vim and spm were from 11,3 and4 strains. However, 3 strains with positive E test results showed negative ampification by all primer pairs. Conclusion：The major MBL genetype of Pseudomonas aeruginosa in 301 hospital and Xinjiang areas are IMP.

关 键 词：假单胞菌 铜绿 金属Β-内酰胺酶 基因型 

分 类 号：R378[医药卫生—病原生物学]