补肾益脑片对加速衰老小鼠P10/Ta小鼠神经细胞衰老相关基因表达的调节  

Regulation of Aging-Related Genes by Chinese Traditional Medicine Bushen Yinao Pian Water Extract in SAMP10/Ta Mice Neural Cells

作　　者：杨婷[1] 张冲[1] 何新[1] 王锦刚[2] 刘桂生[1] 陈清轩[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所分子发育生物学重点实验室 [2]北京科信必成医药科技发展有限公司,北京100086

出　　处：《中国药学杂志》2007年第6期427-432,共6页Chinese Pharmaceutical Journal

基　　金：国家重大基础研究规划项目(973)(2000016107)

摘　　要：目的研究补肾益脑片抗衰老的分子机制。方法应用DDRT-PCR研究补肾益脑片提取物对SAM(senescence-acceler-ated mouse)P10/Ta小鼠原代培养的神经细胞基因表达的影响。结果发现了10个差异表达片段,分别与下列基因或转录产物同源:小鼠mRNA克隆IMAGE4235872,小鼠RIKENcDNA A730024A03基因mRNA,小鼠血清淀粉样蛋白A3(SAA3),异种核蛋白A0(HNRNP A0),小鼠OVCA1(Dph211)mRNA,蛋白酶体26S亚单位,ATP依赖线粒体RNA螺旋酶,小鼠线粒体核蛋白S31(Mrps31),小鼠CX43 gene,小鼠tRNA-Ile.结论补肾益脑片可能通过影响与衰老相关的基因表达发挥抗衰老作用。OBJECTIVE To investigate the anti-ageing molecular mechanism about Bushen Yinao Pian. METHODS The extract of Bushen Yinao Plan was added to the primary culture cells of SAMP10/Ta mouse brain. By using the technique of DDRT-PCR, medicine-specific expression genes were investigated. RESULTS Ten medicine-specific expression genes were found. Sequencing analysis showed that most of those fragments were homologous with some of certain gene cDNA that were related with senile. The fragments were homologous to the genes as follows： Mus musculus, clone IMAGE： 4235872, mRNA; Mus musculus RIKEN cDNA A730024A03 gene （A730024A03Rik）, mRNA; Mouse mRNA fragment for serum amyloid A （SAA） 3 protein; Heterogeneous Nucle- ar Ribonucleoprotein A0 （HNRNP A0） homolog; Mus musculus OVCA1 （Dph211 ） mRNA; proteasome（ prosome, macropain） 26S subunit, non-ATPase, 11 ; ATP-DEPENDENT MITOCHONDRIAL RNA HELICASE homolog; Mus musculus rnitochondrial ribosomal protein S31（Mrps31）, mRNA; Mouse CX43 gene; and tRNA-Ile. CONCLUSION Bushen Yinao Pian actually have anti-aging effects by affecting the expression-manners.

关 键 词：补肾益脑片 抗衰老 SAMP10/Ta DDRT-PCR 衰老相关基因 

分 类 号：R967[医药卫生—药理学]