无机磷分解菌BL-11的鉴定及其解磷能力研究  

Studies on the Capacity of Phosphate Dissolving and the Identification of Strain BL-11

作　　者：杜春梅[1] 金术超[1] 王葳[1] 平文祥[1] 杨慧[1] 

机构地区：[1]黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨150080

出　　处：《微生物学通报》2007年第2期283-286,共4页Microbiology China

基　　金：黑龙江省普通高校骨干教师创新能力资助项目(No1054G037)

摘　　要：研究了解磷菌株BL-11的菌体形态、生理生化特性,结合该菌的16SrDNA序列分析结果,将菌株BL-11鉴定为侧孢短芽孢杆菌。菌株BL-11在以Ca3(PO4)2为唯一磷源的培养液中的可溶性磷得率为10.91%;在以砂子为唯一磷源的培养液中,可溶性磷得率为1.56%。分解Ca3(PO4)2的最佳条件为30℃,180r/min,pH7-8;最佳培养基配方为蔗糖20g/L,(NH4)2HCO30.3g/L,MgSO4.7H2O0.5g/L,NaCl0.3g/L,KCl0.5g/L,FeSO40.03g/L,MnSO4.H2O0.03g/L。The morphology, cultural characteristics, physiological and biochemical properties of phosphate solubilizing strain BL-11 were studied. Combine with its 16S rDNA sequence analysis result strain BL-11 was identified as Brevibacillus laterosporus. Results showed that the phosphate solubilizing efficiency of strain BL-11 was up to 10. 91% when with Ca3 （PO3 ）2 as the sole phosphorus source in the /nedium, while the efficiency of that was up to 1.56% when with sands as the sale phosphorus source. An optimal media of BL-11 strain for soluhilizing phosphate was obtained by orthogonal test. It was composed of sugar 20g/L, （ NH4 ） 2 HCO3 0. 3g/L, MgSO4. 7H2O 0. 5g/L, NaCl 0. 3 g/L, KCl 0. 5g/L, FeSO4 0. 03g/L, MnSO4. H2O 0. 03 g/L While other conditions were consisted of initial pH7. 0-8.0, 180 r/min, and 30℃.

关 键 词：菌株BL-11 鉴定 解磷能力 

分 类 号：S154.3[农业科学—土壤学]