ARDRA联合RAPD对不动杆菌基因型鉴定的研究  

Combination of ARDRA and RAPD Genotyping Technique in Acinetobacter spp.Genomic Species Identification

作　　者：张永[1] 陈余清[1] 唐英春[2] 张扣兴[2] 

机构地区：[1]蚌埠医学院附属医院,蚌埠233000 [2]中山大学附属第三医院,广州510630

出　　处：《微生物学通报》2007年第2期303-306,共4页Microbiology China

基　　金：安徽省高校自然科学研究项目(No2006kj49B)

摘　　要：收集多重耐药的不动杆菌10株,以标准参照株作对照,采用扩增核糖体DNA限制性酶切(ARDRA)DNA指纹技术联合随机扩增多态性DNA(RAPD)技术对其基因亚型进行分析;以非加权组间平均法(UPG-MA)进行聚类分析。该法可以有效地鉴定不动杆菌基因亚型;并从10株不动杆菌中鉴定出1株琼氏不动杆菌及9株鲍曼不动杆菌。ARDRA联合RAPD基因指纹分型技术有良好的互补性,可准确鉴定不动杆菌基因型。A total of 10 nonrepetitive multidrug-resistant Acinetobacter strains were collected. With the reference of A, calcoaceticus （ ATCC23055 ） , A. baumanrdi （ATCC19606） A. lwoffii （ ATCC17986 ） and A. junii （ NCTC5866 ） , DNA fingerprint techniques ARDRA （ Amplified ribosomal DNA restriction Analysis） and RAPD （ Random amplified polymorphism DNA） were carried out to identify the genomic species of Acinetobacter spp. The distances between them were calculated by unweighted pair group method with arithmetic （UPGMA） . Genotypes of Acinetobacter spp. can be effectively classified and an A. junii together with nine A. baumannii isolates was genomically identified. The combination of ARDRA and RAPD DNA-fingerprint technique showed high complementarity, it could be a useful tool in Acinetobarter genotnic species identification.

关 键 词：不动杆菌 DNA指纹分型 随机扩增多态性 聚类分析 

分 类 号：R378[医药卫生—病原生物学]