一株不需谷氨酸的产聚γ-谷氨酸菌株的筛选与鉴定  

Screening and Identification of an Independent-glutamic Acid Strain Producing Poly (γ-glutamic acid)

作　　者：施庆珊[1] 李诚斌[1] 王春华[1] 欧阳友生[1] 陈仪本[1] 疏秀林[1] 谢小保[1] 

机构地区：[1]广东省微生物研究所广东省菌种保藏与应用重点实验室,广州510070

出　　处：《微生物学通报》2007年第2期307-311,共5页Microbiology China

基　　金：广东省科技攻关项目(No2005B10401007);广州市自然科学基金(No2005J1-C0051);广州市科技攻关项目(No2006Z3-D0291)

摘　　要：从豆制品中分离得到17株不依赖谷氨酸作为发酵底物的γ-PGA生产菌株,分别以氨盐和葡萄糖作为氮源和碳源的培养基进行好氧发酵,并测定发酵液中PGA的含量,对其中一株PGA高产菌株PGA-O-7进行了形态、生理生化和遗传学研究,结果表明PGA-O-7为枯草芽孢杆菌(Bacillus subtilis)。在以葡萄糖为碳源、硫酸铵为氮源的发酵培养基中,30℃振荡培养3d,PGA产量可达到2.8mg/mL。17 strains of bacterium that produced a large amount of γ-PGA when it was grown aerobically in a culture medium containing ammonium salt and sugar as sources of nitrogen and carbon respectively, were isolated from bean products. With the following identifications of colony morphology, physiological and biochemistry experiments, and genetics, the strain PGA-O-7 was classified as a Bacillus subtilis. The PGA production 2.8 （mg/mL） was obtained when it was grown in a medium containing 3% ammonium sulfate and 4% glucose at 30℃ for 72h with sharking.

关 键 词：聚谷氨酸(γ-PGA) 筛选 鉴定 枯草芽孢杆菌(Bacillus subtilis) 

分 类 号：Q93-3[生物学—微生物学]