大鼠死后肝脏磷酸果糖激酶-2含量的变化  

Postmortem Changes of Liver Phosphofructokinase-2 Level in Rats Following Different Causes of Death

作　　者：金菊[1] 李斌 焦白光[3] 付佳[4] 黄红[1] 宋宇[1] 彭其毅[1] 廖志钢[1] 

机构地区：[1]四川大学华西基础医学与法医学院,四川成都610041 [2]四川华大司法鉴定所,四川成都610041 [3]电子科技大学医院,四川成都610054 [4]四川大学华西公共卫生学院,四川成都610041

出　　处：《法医学杂志》2007年第2期84-85,89,F0002,共4页Journal of Forensic Medicine

摘　　要：目的分析不同死亡时间和死亡原因大鼠肝脏组织内磷酸果糖激酶-2(PFK-2)含量的变化。方法大鼠105只随机分为三组,以不同方式(失血、窒息、断颈)处死。分别于死后0、1、2、4、8、12和24h取大鼠肝脏组织,免疫组化和图像分析技术测定大鼠PFK-2变化。结果三组不同死亡原因大鼠肝脏组织内PFK-2的含量随死亡时间延长呈规律性减弱趋势。结论大鼠肝脏组织内PFK-2的变化可以反映死亡时间的变化趋势。Objective To study the changes of liver phosphofructokinase-2 （PFK-2） level at different postmortem intervals as well as due to different causes of death. Methods One hundred and five rats were randomly divided into 3 groups and the rats were sacrificed by either bleeding, suffocating, and neck breaking, respectively. The content of liver PFK-2 at 0, 1, 2, 4, 8, 12 and 24 hours following death were studied using immunohistochemishtry and image analysis. Results PFK-2 content of the rat＇s liver in all 3 groups showed a linear decrease at different postmortem intervals with no significant statistical differences found between the different groups. Conclusion PFK-2 level in rat liver appears to decrease linearly in correlation with prolonged PMI.

关 键 词：死亡时间 磷酸果糖激酶-2 含量 肝组织 

分 类 号：D919.1[医药卫生—法医学] Q55[政治法律—法学]