荧光免疫标记抗体法测定血痕ABO血型  

Detecting ABO Blood Type of Bloodstain with Fluorescent Antibody Method

作　　者：王瑞恒[1] 闫立强[2] 于卫建[3] 刘效巍[4] 曲淑贤[5] 

机构地区：[1]辽宁师范大学法学院,辽宁大连116029 [2]辽宁警官高等专科学校,辽宁大连116033 [3]大连市红十字血液中心,辽宁大连116001 [4]大连医科大学附属第一医院,辽宁大连116003 [5]大连医科大学分子生物学重点实验室,辽宁大连116020

出　　处：《法医学杂志》2007年第2期127-129,F0004,共4页Journal of Forensic Medicine

基　　金：辽宁省教育厅项目(2005L204);大连市科技局科技攻关项目(2004B3SF156)

摘　　要：目的探讨采用荧光免疫标记抗体法测定血痕血型的可行性和优点。方法根据抗原抗体特异性结合的原理,首先对抗A、抗B单克隆抗体进行荧光标记,然后使荧光标记抗体与相应抗原(血痕)在最佳条件下结合,最后荧光显微镜镜检,判定血痕的血型。结果采用该方法测定血痕的血型结果准确、灵敏度高、操作简单、检验时间短。结论该方法可以作为一种测定血痕血型的检验方法使用。Objective To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material. Methods According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody （MA） and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies （FLA） were bound with corresponding biological material （such as bloodstain） in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent. Results The fluorescent antibody method is highly sensitive, accurate and simple. Conclusion The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.

关 键 词：荧光免疫标记抗体法 血痕 ABO血型 法医物证学 

分 类 号：D919.2[医药卫生—法医学]