6种淫羊藿黄酮抗氧化和抗肿瘤活性的比较  

Comparison of antioxidative and antitumor activities of six flavonoids from Epimedium koreanum

作　　者：王婷[1] 张金超[2] 陈瑶[2] 黄锋[1] 杨梦甦[2] 肖培根[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094 [2]香港城市大学深圳研究院

出　　处：《中国中药杂志》2007年第8期715-718,共4页China Journal of Chinese Materia Medica

基　　金：国家863计划资助项目(2003AA2Z2052)

摘　　要：目的：初步探讨淫羊藿黄酮的抗氧化活性与其化学结构的关系以及抗肿瘤作用与取代基的关系。方法：利用硅胶和凝胶柱色谱分离淫羊藿中单体黄酮成分，根据化合物光谱数据（ESI-MS，^1HNMR和^13CNMR）鉴定其结构，利用MTT法和DPPH法测定6个单体黄酮的细胞毒性和抗氧化清除自由基的能力。结果：从地上部分的醋酸乙酯萃取物中分离得到6个化合物，分别为淫羊藿苷（Ⅰ），木犀草素（Ⅱ），宝藿苷Ⅱ（Ⅲ），金丝桃苷（Ⅳ），朝藿素B（V）及宝藿苷Ⅰ（Ⅵ）。活性研究结果表明，在3．125—200μmol·L^-1剂量，化合物Ⅰ，Ⅲ和Ⅵ对DPPH自由基基本上没有清除作用，而化合物Ⅱ，Ⅳ和V对DPPH则显示出了较好的自由基清除作用，并随浓度的增加清除作用增强，其作用强度明显好于维生素C；化合物Ⅰ，Ⅱ，V和Ⅵ对人乳腺癌细胞株（MCF-7）和人肝癌细胞株（HepG2）的生长均表现出增殖抑制作用，而化合物Ⅲ和Ⅳ对2种细胞株的生长则基本没有影响。结论：B环是黄酮类物质抗氧化、清除自由基的主要活性部位；黄酮8-位异戊烯基取代不是其抗肿瘤活性的决定因素。Objective： To study the antioxidative and antitumor activities of flavonoids isolated from Epimedium koreanum. Method： The compounds were separated by column chromatography with silica gel and Sephadex LH-20, and identified by spectral analysis （ESI-MS, ^1H-NMR and ^13C-NMR） respectively. DPPH radical scavenging assay and MTY assay were used to observe the antioxidative and antitumor abilities. Result： Six compounds were isolated from the the ethyl acetate extract of the aerial part. Their structures were identified as icariin （ Ⅰ ）, luteolin （ Ⅱ ）, baohuoside Ⅱ （Ⅲ）, hyperoside （ Ⅳ）, epimedokoreanin B （ V ） and baohuoside Ⅰ（Ⅵ）. The results indicated that at concentrations of 3. 125 -200 μmol· L^-1, compound Ⅰ , Ⅲ and Ⅵ had no ability to scavenge the DPPH radical, but the scavenging ability of compounds Ⅱ, Ⅳ and V were stronger than that of Vit c in dose-dependant manner. Compounds Ⅰ , Ⅱ , V and Ⅵ could inhibit the proliferation of MCF-7 and HepG2 in dose-dependant manner, but compounds Ill and IV had no effect on the proliferation. Conclusion： The antitumor activity of E. koreanum may be partially related to the antioxidantive activity of flavonoids.

关 键 词：淫羊藿 黄酮 抗氧化 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]