黑龙江膜荚黄芪多糖提取与含量测定  

Extraction and Determination of Polysaccharide from Astragalus Membranaceus in Heilongjiang Province

作　　者：李宏岩[1] 张莹[1] 彭茵[1] 

机构地区：[1]大连医科大学检验医学院,辽宁省大连市116027

出　　处：《光谱实验室》2007年第2期135-137,共3页Chinese Journal of Spectroscopy Laboratory

摘　　要：研究了水提醇沉法制备的黄芪多糖提取物,采用苯酚-硫酸比色法测定多糖的含量,在0.00—0.14mg/mL线性范围内具有良好的线性关系,本法的RSD为0.98%。回收率在94.8%—103.5%之间。测定黄芪多糖含量为4.3%。此方法显色快速,反应稳定性较好,准确度、精密度较高。The crude astragalus in Astragalus membranaceus from Heilongjiang was extracted by water and purifying of EtOH. The content of Polysaccharides in Astragalus Membranaceus（Fisch） was determined by Phenol-sulfuric acid method with a good recovery of 94.8%-103.5%.

关 键 词：黄芪 多糖 苯酚-硫酸比色法 

分 类 号：O657.32[理学—分析化学]