PHA分光光度法测定凉山烟叶中镁的研究  

Study on PHA-Spectrophotometric Determination of Magnesium in Liangshan Leaf Tobacco

作　　者：张万明[1] 张林[1] 罗茜[1] 马金华[1] 蒋中国[1] 

机构地区：[1]西昌学院生化系,四川省西昌市615022

出　　处：《光谱实验室》2007年第2期145-146,共2页Chinese Journal of Spectroscopy Laboratory

摘　　要：在pH=7.0 NH4Ac-HAc缓冲介质中,镁与苦氨酸偶氮-H-酸(PHA)生成1∶2蓝紫色络合物,λmax=632nm,ε=1.2×104L.mol-1.cm-1,镁的含量在1.0—80μg/50mL内符合比耳定律,本方法用于烟草样品中镁的测定,结果令人满意。In pH=7.0 NH4Ac-HAc buffer solution,magnesium can react with picramazo-H-acid to form a 1 ： 2 blue complex. The molar absorptivity is 1.2 × 10^4L · mol^-1· cm^-1 at 632nm. Beer＇s law is obeyed in the range of 1.0-80μ0g/50mL This method can be applied to the determination of magnesium in liangshan leaf tobacco with satisfactory results.

关 键 词：苦氨酸偶氮-H-酸 镁 分光光度法 

分 类 号：O657.32[理学—分析化学]