双酚A与人血清白蛋白作用特征的热力学研究  

Thermodynamic Studies on the Binding Feature between BPA and Human Serum Albumin

作　　者：李少旦[1] 黎明强[1] 蒙进怀[1] 覃华菁[1] 罗正华 卢小玲[1] 覃芸[1] 宁玉华[1] 李德洁[1] 李华茜[1] 

机构地区：[1]广西柳州市疾病预防控制中心,广西柳州市545001

出　　处：《光谱实验室》2007年第2期161-164,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：广西壮族自治区卫生厅自筹经费立项课题;课题编号:Z2006307

摘　　要：利用荧光光谱和吸收光谱法研究了双酚A与人血清白蛋白结合反应的特征,实验结果表明,双酚A对人血白蛋白有较强的荧光猝灭作用,根据荧光猝灭数据和非辐射能量转移机理,由Sterm-Volmer和Lineweaver-Burk方程处理实验数据,得到了结合反应的结合常数、结合位置和结合过程的基本热力学参数。The characteristics of the binding reaction of biphenol A （BPA） with Human Serum Albumin （HAS） was investigated by fluorescence and absorption spectra. The BPA exhibits a strong quenching effect on the fluorescence of HSA,through a nonradiative energy transfer. The data of fluorescence quenching were analyzed according to Sterm-Volmer and Lineweaver-Burk equation,the binding constant of the reaction, the position of binding and the basic thermodynamic parameters of the binding process were obtained, respectively.

关 键 词：双酚A 人血清白蛋白 热力学参数 

分 类 号：O657.32[理学—分析化学]