LC-MS/MS法测定鱼肉中氯霉素残留  

Determination of chloramphenicol residues in fish by liquid chromatography-tandem mass spectrometry

作　　者：王新丽[1] 

机构地区：[1]沈阳市铁西区疾病预防控制中心,沈阳110000

出　　处：《中国卫生检验杂志》2007年第3期456-457,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立鱼肉中氯霉素残留的液相色谱-串联质谱检测方法。方法：以氘代氯霉素为内标，乙酸乙酯超声振荡提取鱼肉组织中氯霉素，正己烷除去其中的脂肪，LC—MS／MS测定。结果：氯霉素检测的线性范围0．1—50μμ／L，／．2=0．9982。加标水平为0．5、2．5μg／kg时，平均回收率大于96％，相对标准偏差小于14％。鱼肉中氯霉素的定量检测下限为0．025μg／kg。结论：本法快速、灵敏，操作简便，检测结果准确，适用于鱼肉中氯霉素残留的检测。Objective： A liquid chromatography -tandem mass spectrometry method was developed for the determination of chloramphenieol residues in fish. Methods：The analyte and the deuterated internal standard（ d5 -CAP） were extracted with ethyl acetate and cleaned up by n - hexane, detected with tandem mass spectrometry. Results：Calibration curves of chloramphenicol were linear in the rang of 0. 1 - 50 μg/L（ r^2 = 0. 9982）. At the spiking levels of 0. 5,2. 5 μg/kg, the mean recovery was more than 96% and the relative standard derivations were less than 14%. The limit of quantification for chloramphenicol in fish was 0. 025 μg/kg. Conclusion：The method is rapid, sensitive, specific and suitable for determination of ehloramphenieol residues in fish.

关 键 词：液相色谱-串联质谱法(LC—MS/MS) 鱼 氯霉素 残留 

分 类 号：O657.63[理学—分析化学]