高效液相色谱法同时测定化妆品中地塞米松和泼尼松  

Simultaneous determination of dexamethasone and prednisone in cosmetic by reversed-phase liquid chromatography with ultraviolet detection

作　　者：贾薇[1] 宋鸣 于晓英[1] 张秀丽[1] 张玉黔[1] 

机构地区：[1]沈阳市疾病预防控制中心,沈阳110031 [2]辽宁省刑事科学技术研究所,沈阳110032

出　　处：《中国卫生检验杂志》2007年第3期463-464,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立高效液相色谱法测定化妆品中地塞米松和泼尼松的方法。方法：醋酸氟氢可的松为内标，乙腈-水-乙酸铵溶液为流动相，甾体激素经C18色谱柱反相液相色谱分离紫外检测。结果：被测两组分浓度在5—200μg／ml范围呈良好线性关系，回收率分别为95．9％和94．7％，相对标准偏差分别为4．55％和4．11％。结论：本方法准确可靠，适合化妆品中地塞米松及泼尼松的含量测定。Objective：To develop a method for the determination of dexamethasone and prednisone in cosmetic by high performance liquid chromatography. Methods：The steroids were analyzed isocratically by reversed - phase HPLC with an octadecylsilane -bonded （ODS） column using ultraviolet detection by employing acetonitrile -water- ammonium acetate solution as mobile phase. The internal standard for the analyses was fludrocortisone acetate. Results：The standard curves were linear over the range of 5 - 200 μg/ml. The recovery was 95.9% and 94. 7%, respectively and the RSD were 4. 55% and 4. 11%, respectively. Conclusion ：The method is accurate and reliable.

关 键 词：地塞米松 泼尼松 化妆品 高效液相色谱 

分 类 号：O657.72[理学—分析化学]